Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway

doi:10.1002/ptr.6367

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway
	Li, Cong 1; Xu, Huanli 1; Chen, Xiaoliang 2; Chen, Jijun3 ; Li, Xiao 1; Qiao, Gan 1; Tian, Ye 1; Yuan, Ru 1; Su, Shuonan 1; Liu, Xiaohui 1
刊名	PHYTOTHERAPY RESEARCH
	2019-07-01
卷号	33 期号:7 页码:1794-1804
关键词	AMPK anticancer aqueous extract of clove autophagy traditional Chinese medicine
ISSN号	0951-418X
DOI	10.1002/ptr.6367
通讯作者	Lin, Xiukun(xiukunlin@126.com)
英文摘要	Cloves (Syzygium aromaticum), a traditional Chinese medicinal herb, displays broad biological activity. In the present study, the aqueous extract of clove (AEC) was prepared, and its anticancer affects were studied. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetra-zolium (MTS) analysis revealed that AEC was able to inhibit cancer cell growth in vitro on several cancer cell lines; the IC50 is around 150 mu g/ml for human pancreatic ASPC-1 and human colon HT-29 cancer cells. Treatment of the cancer cells with AEC also diminished the colony formation significantly in both human pancreatic ASPC-1 cancer cells and human colon HT-29 cancer cells. In vivo study revealed that AEC inhibited the tumor growth significantly in HT-29 xenograft mice model. Transmission electron microscope, flow cytometry assay, and fluorescence microscope analysis confirmed that AEC is capable of inducing cell autophagy. Further study showed that AMPK/ULK pathway plays an important role in AEC-induced autophagy in cancer cells. Analysis of AEC components was performed by liquid chromatograph mass spectrometer approach, and more than nine constitutes were identified in AEC fraction. The study provides evidence that AEC has potential to be developed as a novel anticancer agent or as an adjuvant in cancer chemotherapy.
WOS研究方向	Pharmacology & Pharmacy
语种	英语
WOS记录号	WOS:000477912800005
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/68126]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Lin, Xiukun
作者单位	1.Capital Med Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100069, Peoples R China 2.Shanxi Datong Univ, Basic Med Sch, Datong, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Li, Cong,Xu, Huanli,Chen, Xiaoliang,et al. Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway[J]. PHYTOTHERAPY RESEARCH,2019,33(7):1794-1804.
APA	Li, Cong.,Xu, Huanli.,Chen, Xiaoliang.,Chen, Jijun.,Li, Xiao.,...&Lin, Xiukun.(2019).Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway.PHYTOTHERAPY RESEARCH,33(7),1794-1804.
MLA	Li, Cong,et al."Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway".PHYTOTHERAPY RESEARCH 33.7(2019):1794-1804.