Transfection reagent Lipofectamine triggers type I interferon signaling activation in macrophages

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 病原菌感染与宿主免疫机制

	Transfection reagent Lipofectamine triggers type I interferon signaling activation in macrophages
	Guo, XM; Wang, H; Li, Y; Leng, XP; Huang, WW; Ma, YB; Xu, T; Qi, XP
刊名	IMMUNOLOGY AND CELL BIOLOGY
	2019
卷号	97 期号:1 页码:92-96
ISSN号	0818-9641
英文摘要	The commercial transfection reagent Lipofectamine has been widely used for cytoplasmic delivery of nucleic acids and for cytosolic engagement with intracellular innate immune sensors to trigger type I interferon (IFN) production. However, the effect of Lipofectamine alone on type I IFN response has not been studied in detail. Here, we show that Lipofectamine induced type I IFN signaling in both RAW 264.7 macrophage-like cells and primary bone marrow-derived macrophages. Type I IFN induction was dependent on interferon regulatory factor (IRF)3 and IRF7 and partially required the toll/interleukin-1 receptor-domain-containing adapter-inducing interferon-beta. In contrast, the transfection reagent Xfect did not activate type I IFN signaling. Our study highlights the potential confounding experimental interpretation when using Lipofectamine-based transfection for delivering intracellular ligands and provides important insights into lipid signaling in innate immune responses.
语种	英语
内容类型	期刊论文
源URL	[http://ir.kiz.ac.cn:8080/handle/152453/12535]
专题	昆明动物研究所_病原菌感染与宿主免疫机制
推荐引用方式 GB/T 7714	Guo, XM,Wang, H,Li, Y,et al. Transfection reagent Lipofectamine triggers type I interferon signaling activation in macrophages[J]. IMMUNOLOGY AND CELL BIOLOGY,2019,97(1):92-96.
APA	Guo, XM.,Wang, H.,Li, Y.,Leng, XP.,Huang, WW.,...&Qi, XP.(2019).Transfection reagent Lipofectamine triggers type I interferon signaling activation in macrophages.IMMUNOLOGY AND CELL BIOLOGY,97(1),92-96.
MLA	Guo, XM,et al."Transfection reagent Lipofectamine triggers type I interferon signaling activation in macrophages".IMMUNOLOGY AND CELL BIOLOGY 97.1(2019):92-96.