High concentration of sugars is genotoxic to folate-deficient cells

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 肿瘤生物学

	High concentration of sugars is genotoxic to folate-deficient cells
	Guo, XH; Dai, XQ; Ni, J; Cao, N; Yang, GF; Xue, JL; Wang, X
刊名	MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
	2019
卷号	814 页码:15-22
ISSN号	0027-5107
英文摘要	Patients with type 2 diabetes mellitus (T2DM) are associated with an elevated, but poorly understood baseline of genomic instability (GIN). Expert panels are still debating on whether hyperglycemia is the key element in conferring this high GIN. Since high blood glucose and low blood folate are prevalent in T2DM, we hypothesized that high glucose may work with low folate to induce GIN. Using NCM460, CCD841 and L02 cell lines as in vitro cell models, we investigated the genotoxic effects of high sugars (HS; 1-2% glucose, fructose, galactose or sucrose) alone or in combination with folate deficiency (23 nM, FD) over a course of 7 days by the cytokinesis block micronucleus assay. We found that HS is nongenotoxic to NCM460, CCD841 and L02 cells. However, the combination of HS and FD induced significantly higher levels of micronuclei, nucleoplasmic bridges and nuclear buds. Our in vitro work demonstrates that HS is non-genotoxic under folate repletive condition, but is genotoxic under FD condition. These results provide preclinal proof of concept that concomitant hyperglycemia and low folate may explain, at least in part, the high baseline of GIN in T2DM patients, suggesting that folate levels should be kept under control in order to limit the risk of GIN and carcinogenesis in T2DM.
语种	英语
内容类型	期刊论文
源URL	[http://ir.kiz.ac.cn:8080/handle/152453/12529]
专题	昆明动物研究所_肿瘤生物学
推荐引用方式 GB/T 7714	Guo, XH,Dai, XQ,Ni, J,et al. High concentration of sugars is genotoxic to folate-deficient cells[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2019,814:15-22.
APA	Guo, XH.,Dai, XQ.,Ni, J.,Cao, N.,Yang, GF.,...&Wang, X.(2019).High concentration of sugars is genotoxic to folate-deficient cells.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,814,15-22.
MLA	Guo, XH,et al."High concentration of sugars is genotoxic to folate-deficient cells".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 814(2019):15-22.