Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios

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	Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios
	ZHANG QI ; WU J ; QIAN JIANGCHAO ; CHU J ; ZHUANG Y ; ZHANG S ; Liu W(刘文)
刊名	Lett. Appl. Microbiol.
	2011
卷号	52 期号:2 页码:129-137
ISSN号	0266-8254
其他题名	Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios
通讯作者	QIAN JIANGCHAO ; 刘文
英文摘要	Aims: To overproduce erythromycin C, B or D and evaluate the effect of disruption of tailoring genes eryK and eryG in an industrial erythromycin producer. Methods and Results: The tailoring genes eryG and eryK were inactivated individually or simultaneously by targeted gene disruption in an industrial strain Saccharopolyspora erythraea HL3168 E3, resulting in the overproduction of erythromycin C (2 center dot 48 g l-1), B (1 center dot 70 g l-1) or D (2 center dot 15 g l-1) in the mutant strain QL-G, QL-K or QL-KG, respectively. Analysis of the erythromycin congeners throughout the fermentation indicated that, at the end of fermentation, comparatively large amount of erythromycin D (0 center dot 67 g l-1) was accumulated in QL-G, whereas only small amount of erythromycin D (0 center dot 10 g l-1) was produced in QL-K. Conclusions: Inactivation of tailoring genes eryG and eryK in the high producer did not affect the biosynthesis of erythromycin. However, erythromycin D could be more efficiently methylated by EryG than be hydroxylated by EryK. Significance and Impact of the Study: Development of the mutant strains provides a method for the economical large-scale production of potent lead compounds. The information about the accumulation and conversion of erythromycins in the industrial strains may contribute to further improving erythromycin production.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1111/j.1472-765X.2010.02973.x
语种	英语
WOS记录号	WOS:000286003400007
公开日期	2013-08-21
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/27815]
专题	上海有机化学研究所_上海有机化学研究所
推荐引用方式 GB/T 7714	ZHANG QI,WU J,QIAN JIANGCHAO,et al. Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios[J]. Lett. Appl. Microbiol.,2011,52(2):129-137.
APA	ZHANG QI.,WU J.,QIAN JIANGCHAO.,CHU J.,ZHUANG Y.,...&刘文.(2011).Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios.Lett. Appl. Microbiol.,52(2),129-137.
MLA	ZHANG QI,et al."Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios".Lett. Appl. Microbiol. 52.2(2011):129-137.