Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides

doi:10.1007/s00216-019-01770-w

	Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides
	Hu, Yechen 1,2; Li, Yang 1,2; Gao, Hang 1,2; Jiang, Bo 1; Zhang, Xiaodan 1; Li, Xiao 1; Wu, Qiong 1,2; Liang, Zhen 1; Zhang, Lihua 1; Zhang, Yukui 1
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2019-07-01
卷号	411 期号:18 页码:4159-4166
关键词	Phosphorylated lysine peptides Cleavable hydrophobic derivatization N-Phosphorylation Proteomics Liquid chromatography-tandem mass spectrometry
ISSN号	1618-2642
DOI	10.1007/s00216-019-01770-w
通讯作者	Zhang, Lihua(lihuazhang@dicp.ac.cn)
英文摘要	Because of structural flexibility and acid lability, the identification of phosphorylated lysine (pLys) peptides is a great challenge. We report here a cleavable hydrophobic derivatization (CHD) strategy for the enrichment and identification of pLys peptides. First, 2,5-dioxopyrrolidin-1-yl-3-(decyldisulfanyl)propanoate was synthesized to react with dephosphorylated lysine peptides, and then the derived peptides were captured by a C-18 column, followed by cleavage of the hydrophobic chain, with the specific label left on the target peptides for further identification. By CHD, the enrichment of pLys peptides from interfering peptides (1:1000 mass ratio) was achieved. Furthermore, CHD was applied to screen the pLys targets from Escherichia coli lysates, and 39 pLys sites from 35 proteins were identified. Gene Ontology (GO) analysis showed that these proteins played vital roles in catabolism, metabolism, biogenesis, and biosynthetic processes. All these results demonstrate that CHD might pave the way for comprehensive profiling of the pLys proteome.
资助项目	National Key Research and Development Program of China[2017YFA0505003] ; National Natural Science Foundation[91543201] ; National Natural Science Foundation[21505133] ; National Natural Science Foundation[21725506] ; Chinese Academy of Sciences Key Project in Frontier Science[QYZDY-SSW-SLH017] ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key[DICP TMSR201601]
WOS关键词	BOVINE LIVER ; 1-32P-PHOSPHOHISTIDINE 3-32P-PHOSPHOHISTIDINE ; PROTEIN ; 3-PHOSPHOHISTIDINE ; 6-PHOSPHOLYSINE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000474266400020
资助机构	National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences Key Project in Frontier Science ; Chinese Academy of Sciences Key Project in Frontier Science ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences Key Project in Frontier Science ; Chinese Academy of Sciences Key Project in Frontier Science ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences Key Project in Frontier Science ; Chinese Academy of Sciences Key Project in Frontier Science ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences Key Project in Frontier Science ; Chinese Academy of Sciences Key Project in Frontier Science ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/175434]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang, Lihua
作者单位	1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&D Ctr, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yechen,Li, Yang,Gao, Hang,et al. Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(18):4159-4166.
APA	Hu, Yechen.,Li, Yang.,Gao, Hang.,Jiang, Bo.,Zhang, Xiaodan.,...&Zhang, Yukui.(2019).Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(18),4159-4166.
MLA	Hu, Yechen,et al."Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.18(2019):4159-4166.