Identification and expression analysis of critical microRNA-transcription factor regulatory modules related to seed development and oil accumulation in developing Hippophae rhamnoides seeds

doi:10.1016/j.indcrop.2019.05.011

	Identification and expression analysis of critical microRNA-transcription factor regulatory modules related to seed development and oil accumulation in developing Hippophae rhamnoides seeds
	Li, Jingbin 1,2; Ding, Jian 1; Yu, Xue 1; Li, He 1; Ruan, Chengjiang 1
刊名	INDUSTRIAL CROPS AND PRODUCTS
	2019-10-01
卷号	137 页码:33-42
关键词	Sea buckthorn Transcription factor miRNA Regulatory network Seed development Oil accumulation
ISSN号	0926-6690
DOI	10.1016/j.indcrop.2019.05.011
通讯作者	Ruan, Chengjiang(ruan@dlnu.edu.cn)
英文摘要	Sea buckthorn (Hippophae rhamnoides) seed oil is rich in omega fatty acids and various other bioactive compounds, which not only have high antioxidant activity but may also prevent cardiovascular diseases. However, the molecular mechanisms of oil biosynthesis and fatty acid accumulation in developing sea buckthorn seeds remain unknown. Altering the levels of certain miRNAs and transcription factors can affect the expression of multiple genes involved in seed development and fatty acid biosynthesis, resulting in changed seed oil contents. Considering that previous studies have focused mainly on these two regulators separately, we attempted to combine the study of miRNAs and transcription factors to construct a coregulatory network. In the present study, we generated cDNA libraries and small RNA libraries from the seeds of high-oil-content and low-oil-content sea buckthorn cultivars at four developmental stages, and then used high-throughput sequencing technology and bioinformatics methods to identify 140 differentially expressed transcription factors, 137 known miRNAs, and 250 novel miRNAs. Through target relationship prediction, 31 potential transcription factors were found to be associated with 27 miRNAs in 18 independent networks, showing the multiplicity of the regulators. In particular, 9 miRNAs and 5 transcription factors were found as hubs in the network. Furthermore, we hypothesized that 12 regulatory modules might play combinatory regulatory roles in seed development and oil accumulation. In developing seeds, the expression levels of these miRNAs and transcription factors were significantly different between the two cultivars. Taken together, these data describe the first miRNA-transcription factor coregulatory network in developing seeds of sea buckthorn. These findings will be helpful for understanding seed development and oil accumulation at the molecular level in sea buckthorn.
资助项目	National Natural Science Foundation of China[31570681] ; Fundamental Research Funds for the Central Universities
WOS关键词	FATTY-ACID BIOSYNTHESIS ; ARABIDOPSIS ; SEEDLINGS ; GENE
WOS研究方向	Agriculture
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000473376800005
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/175414]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Ruan, Chengjiang
作者单位	1.Dalian Minzu Univ, Inst Plant Resources, Minist Educ, Key Lab Biotechnol & Bioresources Utilizat, 18 Liaohe West Rd, Dalian 116600, Liaoning, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Li, Jingbin,Ding, Jian,Yu, Xue,et al. Identification and expression analysis of critical microRNA-transcription factor regulatory modules related to seed development and oil accumulation in developing Hippophae rhamnoides seeds[J]. INDUSTRIAL CROPS AND PRODUCTS,2019,137:33-42.
APA	Li, Jingbin,Ding, Jian,Yu, Xue,Li, He,&Ruan, Chengjiang.(2019).Identification and expression analysis of critical microRNA-transcription factor regulatory modules related to seed development and oil accumulation in developing Hippophae rhamnoides seeds.INDUSTRIAL CROPS AND PRODUCTS,137,33-42.
MLA	Li, Jingbin,et al."Identification and expression analysis of critical microRNA-transcription factor regulatory modules related to seed development and oil accumulation in developing Hippophae rhamnoides seeds".INDUSTRIAL CROPS AND PRODUCTS 137(2019):33-42.