Serum metabolome and targeted bile acid profiling reveals potential novel biomarkers for drug-induced liver injury

doi:10.1097/MD.0000000000016717

	Serum metabolome and targeted bile acid profiling reveals potential novel biomarkers for drug-induced liver injury
	Ma, Zhenhua 1,2; Wang, Xiaomei 2; Yin, Peiyuan ; Wu, Ruihong 2,3; Zhou, Lina 3; Xu, Guowang 3; Niu, Junqi 2
刊名	MEDICINE
	2019-08-01
卷号	98 期号:31 页码:11
关键词	bile acid biomarkers drug induced liver injury serum metabolic profiling UHPLC-MS/MS
ISSN号	0025-7974
DOI	10.1097/MD.0000000000016717
通讯作者	Xu, Guowang(xugw@dicp.ac.cn) ; Niu, Junqi(junqi_niu@163.com)
英文摘要	This study aims to determine the non-invasive, reliable and sensitive biochemical parameters for the diagnosis of drug-induced liver injury (DILI). Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) and selected reaction monitoring (SRM) were used to profile the serum metabolome and quantify 15 targeted bile acid metabolites, respectively, in samples obtained from 38 DILI patients and 30 healthy controls. A comparison of the resulting serum metabolome profiles of the study participants revealed significant differences between DILI patients and healthy controls. Specifically, serum palmitic acid, taurochenodeoxycholic acid, glycocholic acid (GCA), and tauroursodeoxycholic acid (TUDCA) levels were significantly higher, and serum lysophosphatidylethanolamine levels were significantly lower in DILI patients vs healthy controls (P<.001). Furthermore, the SRM assay of bile acids revealed that the increase in GCA, taurocholic acid (TCA), TUDCA, glycochenodeoxycholic acid (GCDCA), glycochenodeoxycholic sulfate (GCDCS), and taurodeoxycholic acid (TDCA) corresponded to a higher degree of liver damage. These results also indicate that serum concentrations of chenodeoxycholic acid (CDCA), deoxycholic acid (DCA) and lithocholic acid (LCA) were significantly lower in patients with severe DILI, when compared to healthy controls, and that this decrease was closely correlated to the severity of liver damage. Taken together, these results demonstrate that bile acids could serve as potential biomarkers for the early diagnosis and severity of DILI.
资助项目	National Basic Research Program of China (973 Program)[2015CB554304] ; National Natural Science Foundation of China[81373057] ; Health and Family Planning Commission Project of Jilin Province[2016Q043]
WOS关键词	HEPATOCELLULAR-CARCINOMA ; CAUSALITY ASSESSMENT ; MASS-SPECTROMETRY ; ADVERSE REACTIONS ; HEPATITIS-B ; MODEL ; METABONOMICS ; INFLAMMATION ; HEPATOCYTES ; VALIDATION
WOS研究方向	General & Internal Medicine
语种	英语
出版者	LIPPINCOTT WILLIAMS & WILKINS
WOS记录号	WOS:000483216200065
资助机构	National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Health and Family Planning Commission Project of Jilin Province ; Health and Family Planning Commission Project of Jilin Province ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Health and Family Planning Commission Project of Jilin Province ; Health and Family Planning Commission Project of Jilin Province ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Health and Family Planning Commission Project of Jilin Province ; Health and Family Planning Commission Project of Jilin Province ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Health and Family Planning Commission Project of Jilin Province ; Health and Family Planning Commission Project of Jilin Province
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/172953]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Xu, Guowang; Niu, Junqi
作者单位	1.Beihua Univ, Affiliated Hosp, Dept Hepatol, Jilin, Jilin, Peoples R China 2.Jilin Univ, Hosp 1, Dept Hepatol, Changchun, Jilin, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Ma, Zhenhua,Wang, Xiaomei,Yin, Peiyuan,et al. Serum metabolome and targeted bile acid profiling reveals potential novel biomarkers for drug-induced liver injury[J]. MEDICINE,2019,98(31):11.
APA	Ma, Zhenhua.,Wang, Xiaomei.,Yin, Peiyuan.,Wu, Ruihong.,Zhou, Lina.,...&Niu, Junqi.(2019).Serum metabolome and targeted bile acid profiling reveals potential novel biomarkers for drug-induced liver injury.MEDICINE,98(31),11.
MLA	Ma, Zhenhua,et al."Serum metabolome and targeted bile acid profiling reveals potential novel biomarkers for drug-induced liver injury".MEDICINE 98.31(2019):11.