Isolation and identification of phosphorylated lysine peptides by retention time difference combining dimethyl labeling strategy

doi:10.1007/s11426-018-9433-3

	Isolation and identification of phosphorylated lysine peptides by retention time difference combining dimethyl labeling strategy
	Hu, Yechen 1,2; Weng, Yejing 1,2; Jiang, Bo 1; Li, Xiao 1; Zhang, Xiaodan 1; Zhao, Baofeng 1; Wu, Qiong 1,2; Liang, Zhen 1; Zhang, Lihua 1; Zhang, Yukui 1
刊名	SCIENCE CHINA-CHEMISTRY
	2019-06-01
卷号	62 期号:6 页码:708-712
关键词	phosphorylated lysine retention time difference dimethyl labeling N-phosphorylation proteome LC-MS/MS
ISSN号	1674-7291
DOI	10.1007/s11426-018-9433-3
通讯作者	Zhang, Lihua(lihuazhang@dicp.ac.cn)
英文摘要	Protein phosphorylation plays essential roles in various biological procedures. Despite the well-established enrichment strategies for O-phosphoproteomics, the intrinsic acid lability of N-P phosphoramidate bond (phosphorylation of histidine, arginine and lysine) has impaired the progress of N-phosphoproteomics. Herein, we reported a retention time difference combining dimethyl labeling (ReDD) strategy for the isolation and identification of phosphorylated lysine (pLys) peptides. By such a method, pLys peptide could be isolated under 100000-fold interference of non-phosphorylated peptides. Furthermore, ReDD strategy was applied to map pLys sites from E. coli samples, leading to the identification of 11 pLys sites, among which K26p that originating from autonomous glycyl radical cofactor was validated both in mass spectrometry and HPLC co-elution experiments. Furthermore, 112 pLys sites from 100 proteins were identified in HeLa cells. All these results demonstrate that ReDD could provide a first glimpse into Lys phosphorylation, and could be an important step toward the global perspective on protein phosphorylation.
资助项目	National Key Research and Development Program of China[2017YFA0505003] ; National Key Research and Development Program of China[2016YFA0501401] ; National Natural Science Foundation of China[21505133] ; National Natural Science Foundation of China[21725506] ; National Natural Science Foundation of China[91543201] ; CAS Key Project in Frontier Science[QYZDY-SSW-SLH017] ; DICP, Chinese Academy of Sciences[DICP TMSR201601]
WOS关键词	LABILE HISTONE PHOSPHATES ; HISTIDINE PHOSPHORYLATION ; ARGININE PHOSPHORYLATION ; ESCHERICHIA-COLI ; CHEMICAL BIOLOGY ; PROTEIN-KINASES ; PHOSPHOHISTIDINE ; PHOSPHOARGININE ; RAT ; PHOSPHOPROTEOME
WOS研究方向	Chemistry
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000470762900008
资助机构	National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; DICP, Chinese Academy of Sciences ; DICP, Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; DICP, Chinese Academy of Sciences ; DICP, Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; DICP, Chinese Academy of Sciences ; DICP, Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Key Project in Frontier Science ; CAS Key Project in Frontier Science ; DICP, Chinese Academy of Sciences ; DICP, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/172036]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang, Lihua
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yechen,Weng, Yejing,Jiang, Bo,et al. Isolation and identification of phosphorylated lysine peptides by retention time difference combining dimethyl labeling strategy[J]. SCIENCE CHINA-CHEMISTRY,2019,62(6):708-712.
APA	Hu, Yechen.,Weng, Yejing.,Jiang, Bo.,Li, Xiao.,Zhang, Xiaodan.,...&Zhang, Yukui.(2019).Isolation and identification of phosphorylated lysine peptides by retention time difference combining dimethyl labeling strategy.SCIENCE CHINA-CHEMISTRY,62(6),708-712.
MLA	Hu, Yechen,et al."Isolation and identification of phosphorylated lysine peptides by retention time difference combining dimethyl labeling strategy".SCIENCE CHINA-CHEMISTRY 62.6(2019):708-712.