Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel)

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 热带海洋环境国家重点实验室(LTO)

	Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel)
	Huang, GY ; Wang, YS ; Ying, GG ; Dang, AC
刊名	TREES-STRUCTURE AND FUNCTION
	2012
卷号	26 期号:5 页码:1537-1544
关键词	Kandelia candel Mangrove species Metal tolerance Metallothionein
ISSN号	0931-1890
通讯作者	huang_gyh@sina.com
中文摘要	Metallothioneins are Cys-rich, low-molecular weight, metal-binding proteins. In this paper, we aimed to evaluate the roles of type 2 metallothionein in Kandelia candel (KcMT2) by analyzing its mRNA expression in leaves in response to heavy metals, as well as the metal-binding properties of its protein. The transcriptional response of KcMT2 gene to different heavy metals (Zn, Cu, Pb and Cd) was measured using real-time quantitative PCR. The results showed that the gene was regulated by the four metals tested. Significant increase in the expression of KcMT2 mRNA was also found in response to Zn, Cu, Pb and Cd. To analyze further the possible roles of KcMT2 against heavy metals, it was expressed in Escherichia coli BL21 as a carboxy-terminal extension of glutathione-S-transferase (GST). The E. coli cells expressing GST-KcMT2 fusion protein showed higher growth compared to the control cells under Zn, Cu, Pb or Cd exposure, and the GST-KcMT2 fusion protein had a stronger binding affinity for Zn, Cu, Pb and Cd than GST alone. Results presented here reveal that KcMT2 may play important roles in the heavy metal homeostasis or detoxification.
学科主题	Forestry
语种	英语
WOS记录号	WOS:000308860500011
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10101]
专题	南海海洋研究所_热带海洋环境国家重点实验室(LTO)
推荐引用方式 GB/T 7714	Huang, GY,Wang, YS,Ying, GG,et al. Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel)[J]. TREES-STRUCTURE AND FUNCTION,2012,26(5):1537-1544.
APA	Huang, GY,Wang, YS,Ying, GG,&Dang, AC.(2012).Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel).TREES-STRUCTURE AND FUNCTION,26(5),1537-1544.
MLA	Huang, GY,et al."Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel)".TREES-STRUCTURE AND FUNCTION 26.5(2012):1537-1544.