Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro

	Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro
	Yang, Cuiqin ; Qin, Yaoguo ; Sun, Xin ; Yuan, Shu ; Lin, Honghui
刊名	NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
	2012
卷号	40 期号:1 页码:107-112
关键词	hyperhydricity leaf reversion tissue culture
ISSN号	0255-965X
通讯作者	Yang, CQ (reprint author), Sichuan Univ, Key Lab Bioresources & Ecoenvironm, State Key Lab Hydraul & Mt River Engn, Minist Educ,Coll Life Sci, Chengdu 610065, Peoples R China.
产权排序	3
英文摘要	An efficient protocol was established for Sedum spectabile Boreau propagation. Various leaf parts were used as explants to regenerate planners, the stem segments of which were cultured for shoot proliferation and plantlet multiplication. The results showed that the leaf base was the optimal explant, as compared to both the middle and the top of leaves, for shoot formation. The highest shoot induction of 88.9% was observed on MS medium supplemented with 0.6 mg/l TDZ and 0.1 mg/l NAA. Hyperhydric leaves obtained in primary culture developed first into abnormal somatic embryos 10 days after subculture, and then into hyperhydric planners after an additional 10 days. The hyperhydric planners reversed to normal planners when plant growth regulators were removed from culture medium. Further, stem segments from reversed plantlets were used for shoot regeneration and root induction. Optimal shoot regeneration was obtained in MS medium containing 0.6 mg/l TDZ with 0.1 mg/l NAA. Root induction and root mean number were all higher on auxin-free medium than on medium containing auxins.
学科主题	Plant Sciences
收录类别	SCI
资助信息	National Nature Science Foundation of China [91017004, 31070210, 31000682, 30970214]; Sichuan Nature Science Foundation [2010JQ0080]
语种	英语
WOS记录号	WOS:000306538900015
公开日期	2013-07-03
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/23834]
专题	成都生物研究所_生态研究
推荐引用方式 GB/T 7714	Yang, Cuiqin,Qin, Yaoguo,Sun, Xin,et al. Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro[J]. NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA,2012,40(1):107-112.
APA	Yang, Cuiqin,Qin, Yaoguo,Sun, Xin,Yuan, Shu,&Lin, Honghui.(2012).Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro.NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA,40(1),107-112.
MLA	Yang, Cuiqin,et al."Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro".NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA 40.1(2012):107-112.