A practical high-throughput screening system for feruloyl esterases: Substrate design and evaluation

	A practical high-throughput screening system for feruloyl esterases: Substrate design and evaluation
	Zhang, Shuai-Bing ; Ma, Xiao-Feng ; Pei, Xiao-Qiong ; Liu, Jing-Yuan ; Shao, Hua-Wu ; Wu, Zhong-Liu
刊名	JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
	2012
卷号	74 期号:1-2 页码:36-40
关键词	Feruloyl esterases High-throughput screening Pichia pastoris 2-Chloro-4-nitrophenol Umbelliferyl
ISSN号	1381-1177
通讯作者	Shao, Hua-Wu ; Wu, ZL (reprint author), Chinese Acad Sci, Chengdu Inst Biol, 9 S Renmin Rd,4th Sect, Chengdu 610041, Sichuan, Peoples R China.
产权排序	1
英文摘要	Feruloyl esterases (FAEs) are a group of important industrial enzymes, which could hydrolyze the ester bonds between hydroxycinnamic acids and arabinose or galactose in hemicellulose present in plant cell walls. To establish a practical high-throughput screening system of feruoyl esterases, two new substrates with modified chromo- or fluoro-phore, 2-chloro-4-nitrophenyl ferulate (CNPF; 1b) and umbelliferyl 5-O-feruolyl-alpha-L-arabinofuranoside (UFA; 2a), were designed and synthesized. Both substrates provided significantly improved signal-to-noise ratio compared with previously known structural analogues. The chromogenic substrate CNPF could be readily adapted to the high-throughput screening of feruloyl esterase A from Aspergillus niger, one of the most studied FAEs, with the coefficient of variance of as low as 10.3% as determined for a single sequence library. (C) 2011 Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Chemistry
收录类别	SCI
资助信息	Chinese Academy of Sciences [KSCX1-YW-11B2]; National Natural Science Foundation of China [20802073, 21072183]; Province Science Foundation of Sichuan, China [08ZQ026-023, 2010SZ0128]
语种	英语
WOS记录号	WOS:000297609900007
公开日期	2013-07-03
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/23938]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Zhang, Shuai-Bing,Ma, Xiao-Feng,Pei, Xiao-Qiong,et al. A practical high-throughput screening system for feruloyl esterases: Substrate design and evaluation[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2012,74(1-2):36-40.
APA	Zhang, Shuai-Bing,Ma, Xiao-Feng,Pei, Xiao-Qiong,Liu, Jing-Yuan,Shao, Hua-Wu,&Wu, Zhong-Liu.(2012).A practical high-throughput screening system for feruloyl esterases: Substrate design and evaluation.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,74(1-2),36-40.
MLA	Zhang, Shuai-Bing,et al."A practical high-throughput screening system for feruloyl esterases: Substrate design and evaluation".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 74.1-2(2012):36-40.