Metabolite profiling of plasma and urine from rats with TNBS-induced acute colitis using UPLC-ESI-QTOF-MS-based metabonomics - a pilot study

	Metabolite profiling of plasma and urine from rats with TNBS-induced acute colitis using UPLC-ESI-QTOF-MS-based metabonomics - a pilot study
	Zhang, Xiaojun ; Choi, Franky F. K. ; Zhou, Yan ; Leung, Feung P. ; Tan, Shun ; Lin, Shuhai ; Xu, Hongxi ; Jia, Wei ; Sung, Joseph J. Y. ; Cai, Zongwei ; Bian, Zhaoxiang
刊名	FEBS JOURNAL
	2012
卷号	279 期号:13 页码:2322-2338
关键词	experimental inflammatory bowel disease metabonomics ultra-performance liquid chromatography time-of-flight mass spectrometry
ISSN号	1742-464X
通讯作者	Bian, ZX (reprint author), Hong Kong Baptist Univ, Sch Chinese Med, Kowloon Tong, Hong Kong, Peoples R China.
产权排序	2
英文摘要	The incidence of inflammatory bowel disease, a relapsing intestinal condition whose precise etiology is still unclear, has continually increased over recent years. Metabolic profiling is an effective method with high sample throughput that can detect and identify potential biomarkers, and thus may be useful in investigating the pathogenesis of inflammatory bowel disease. In this study, using a metabonomics approach, a pilot study based on ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) was performed to characterize the metabolic profile of plasma and urine samples of rats with experimental colitis induced by 2,4,6-trinitrobenzene sulfonic acid. Acquired metabolic profile data were processed by multivariate data analysis for differentiation and screening of potential biomarkers. Five metabolites were identified in urine: two tryptophan metabolites [4-(2-aminophenyl)-2,4-dioxobutanoic acid and 4,6-cihydroxyquinoline], two gut microbial metabolites (phenyl-acetylglycine and p-cresol glucuronide), and the bile acid 12a-hydroxy-3-oxocholadienic acid. Seven metabolites were identified in plasma: three members of the bile acid/alcohol group (cholic acid, 12a-hydroxy-3-oxocholadienic acid and cholestane-3,7,12,24,25-pentol) and four lysophosphatidylcholines [LysoPC(20:4), LysoPC(16:0), LysoPC(18:1) and LysoPC(18:0)]. These metabolites are associated with damage of the intestinal barrier function, microbiota homeostasis, immune modulation and the inflammatory response, and play important roles in the pathogenesis of inflammatory bowel disease. Our results positively support application of the metabonomic approach in study of the pathophysiological mechanism of inflammatory bowel disease.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
资助信息	Hong Kong Jockey Club Institute of Chinese Medicine [JCICM4-07]
语种	英语
WOS记录号	WOS:000305279500007
公开日期	2013-07-03
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/23891]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Zhang, Xiaojun,Choi, Franky F. K.,Zhou, Yan,et al. Metabolite profiling of plasma and urine from rats with TNBS-induced acute colitis using UPLC-ESI-QTOF-MS-based metabonomics - a pilot study[J]. FEBS JOURNAL,2012,279(13):2322-2338.
APA	Zhang, Xiaojun.,Choi, Franky F. K..,Zhou, Yan.,Leung, Feung P..,Tan, Shun.,...&Bian, Zhaoxiang.(2012).Metabolite profiling of plasma and urine from rats with TNBS-induced acute colitis using UPLC-ESI-QTOF-MS-based metabonomics - a pilot study.FEBS JOURNAL,279(13),2322-2338.
MLA	Zhang, Xiaojun,et al."Metabolite profiling of plasma and urine from rats with TNBS-induced acute colitis using UPLC-ESI-QTOF-MS-based metabonomics - a pilot study".FEBS JOURNAL 279.13(2012):2322-2338.