Intracellular microenvironment responsive PEGylated polypeptide nanogels with ionizable cores for efficient doxorubicin loading and triggered release

	Intracellular microenvironment responsive PEGylated polypeptide nanogels with ionizable cores for efficient doxorubicin loading and triggered release
	Shi FH ; Ding JX ; Xiao CS ; Zhuang XL ; He CL ; Chen L ; Chen XS
刊名	journal of materials chemistry
	2012
卷号	22 期号:28 页码:14168-14179
关键词	DRUG-DELIVERY POLY(L-GLUTAMIC ACID) GENE DELIVERY CANCER-CELLS PH CARRIERS THERAPY COPOLYMERS POLYMERS
ISSN号	0959-9428
通讯作者	zhuang xl
中文摘要	two kinds of reduction and ph responsive disulfide-cross-linked poly(ethylene glycol)-polypeptide copolymers were prepared through one-step ring-opening polymerization of gamma-benzyl-l-glutamate n-carboxyanhydride (blg nca) or epsilon-benzyloxycarbonyl-l-lysine n-carboxyanhydride (zll nca) and l-cystine n-carboxyanhydride (lc nca) with amino group terminated monomethoxy poly(ethylene glycol) (mpeg-nh2) as macroinitiator. then, the copolymers were deprotected and dispersed in phosphate buffered saline, yielding peg-polypeptide nanogels. doxorubicin (dox), a model anticancer drug, was effectively loaded into nanogels via electrostatic and hydrophobic interactions. the dox release from all dox-loaded nanogels was accelerated in intracellular reductive and acidic conditions, which controlled by fickian diffusion and nanogels swelling. the enhanced intracellular dox release was observed in glutathione monoester (gsh-oet) pretreated hela cells. dox-loaded nanogels showed higher cellular proliferation inhibition towards gsh-oet pretreated hela and hepg2 cells than to unpretreated or buthionine sulfoximine (bso) pretreated cells. hemolysis tests indicated that nanogels were hemocompatible, and the presence of nanogels could reduce the hemolysis ratio (hr) of dox significantly. these features suggest that the nanogels can efficiently load and deliver dox into tumor cells and enhance the inhibition of cellular proliferation in vitro, providing a favorable platform to construct an efficient drug delivery system for cancer therapy.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000305796300045
公开日期	2013-06-30
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48503]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Shi FH,Ding JX,Xiao CS,et al. Intracellular microenvironment responsive PEGylated polypeptide nanogels with ionizable cores for efficient doxorubicin loading and triggered release[J]. journal of materials chemistry,2012,22(28):14168-14179.
APA	Shi FH.,Ding JX.,Xiao CS.,Zhuang XL.,He CL.,...&Chen XS.(2012).Intracellular microenvironment responsive PEGylated polypeptide nanogels with ionizable cores for efficient doxorubicin loading and triggered release.journal of materials chemistry,22(28),14168-14179.
MLA	Shi FH,et al."Intracellular microenvironment responsive PEGylated polypeptide nanogels with ionizable cores for efficient doxorubicin loading and triggered release".journal of materials chemistry 22.28(2012):14168-14179.