A label-free real time fluorometric assay for protease and inhibitor screening with a released heme

	A label-free real time fluorometric assay for protease and inhibitor screening with a released heme
	Li WY ; Chen J ; Jiao HP ; Zhang QF ; Zhou HP ; Yu C
刊名	chemical communications
	2012
卷号	48 期号:81 页码:10123-10125
关键词	ACETYLCHOLINESTERASE ACTIVITY DRUG DISCOVERY PERYLENE PROBE TRYPSIN
ISSN号	1359-7345
通讯作者	yu c
中文摘要	a label free continuous assay for protease activity and inhibitor screening has been developed. a protease (trypsin) could digest hemoglobin. free heme molecules were released. strong pi-pi stacking and hydrophobic interactions with the perylene probe resulted in efficient quenching of the probe's monomer fluorescence.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000308827600014
公开日期	2013-06-30
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48470]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li WY,Chen J,Jiao HP,et al. A label-free real time fluorometric assay for protease and inhibitor screening with a released heme[J]. chemical communications,2012,48(81):10123-10125.
APA	Li WY,Chen J,Jiao HP,Zhang QF,Zhou HP,&Yu C.(2012).A label-free real time fluorometric assay for protease and inhibitor screening with a released heme.chemical communications,48(81),10123-10125.
MLA	Li WY,et al."A label-free real time fluorometric assay for protease and inhibitor screening with a released heme".chemical communications 48.81(2012):10123-10125.