Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products
	Li Wen-gui 1; Dai Fei-yan 1; Cheng Yong-xian 2; Yin Ge-fen 1; Bi Jun-long 1; Li Dian-peng 3
刊名	CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
	2013-04-01
卷号	29 期号:2 页码:290-293
关键词	Porcine reproductive and respiratory syndrome virus(PRRSV) Natural product Cytopathic effect Antiviral activity
ISSN号	1005-9040
通讯作者	Cheng, YX (reprint author), Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650204, Peoples R China. ; yxcheng@mail.kib.ac.cn
英文摘要	Porcine reproductive and respiratory syndrome(PRRS) caused by porcine reproductive and respiratory syndrome virus(PRRSV) is one of the most infectious diseases in the swine industry worldwide, causing big economic losses. Vaccines are major weapons against PRRSV, however, current available vaccines have several limitations. Developing chemical drugs as alternatives is required. On the basis of traditional medical knowledge, we purposely selected 15 natural products originated from Chinese herbs with anti-infectious effects. Their antiviral activities were evaluated by PRRSV-induced cytopathic effect(CPE) on MARC-145 cells and reverse transcription polymerase chain reaction(RT-PCR) assay. Compounds ethoxysanguinarine(EOSG) and atractylodinol were found to be the hits which could significantly reduce PRRSV-associated CPE with 50% inhibited concentration(IC50) values of 7.9 and 39.4 mu mol/L, respectively. Meanwhile, compounds ethoxysanguinarine and atractylodinol significantly decreased mRNA expression of ORF7 gene in a dose-dependent manner. Study results suggest that compounds ethoxysanguinarine and atractylodinol may be useful anti-PRRSV drugs for swine industry or the hits for further lead optimization.
学科主题	Chemistry
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	SYNDROME PRRS ; SANGUINARINE ; TRANSMISSION ; DISCOVERY ; PLANTS ; PIGS
收录类别	SCI
资助信息	Project of Talent Scholarship for the Youth of Yunnan Province of China [2007PY01-48]; Sino-German International Collaboration Project from Yunnan Province of China [2009AC011]; Project of Natural Compound Library Construction from Chinese Academy of Sciences [KSCX2-EW-R-15]; Yunnan Provincial Program for Excellent Scientists, China [2009CI125]
语种	英语
WOS记录号	WOS:000317165900021
公开日期	2013-06-08
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/16469]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming 650201, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650204, Peoples R China 3.Guangxi Inst Bot, Guangxi Key Lab Funct Phytochem Res & Utilizat, Guilin 541006, Peoples R China
推荐引用方式 GB/T 7714	Li Wen-gui,Dai Fei-yan,Cheng Yong-xian,et al. Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2013,29(2):290-293.
APA	Li Wen-gui,Dai Fei-yan,Cheng Yong-xian,Yin Ge-fen,Bi Jun-long,&Li Dian-peng.(2013).Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,29(2),290-293.
MLA	Li Wen-gui,et al."Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 29.2(2013):290-293.