Aptamer carbon nanodot sandwich used for fluorescent detection of protein

	Aptamer carbon nanodot sandwich used for fluorescent detection of protein
	Xu BL ; Zhao CQ ; Wei WL ; Ren JS ; Miyoshi D ; Sugimoto N ; Qu XG
刊名	analyst
	2012
卷号	137 期号:23 页码:5483-5486
关键词	LUMINESCENT QUANTUM DOTS THROMBIN DETECTION BIND NANOCRYSTALS MOLECULES SELECTION PROBES SENSOR CHIP
ISSN号	0003-2654
通讯作者	qu xg
中文摘要	carbon nanodots (c-dots) have attracted growing interest in recent years due to their low cost, ready scalability, excellent chemical stability, biocompatibility, colloidal stability, and resilience of photoluminescence. they have been employed as novel, ideal fluorescent probes for bio-imaging and smart sensing. in addition, taking advantage of their low-cytotoxicity, c-dots have potential applications in biochemical and cell biological fields. herein, we present the first assay with aptamer-functionalized c-dots as a sensory platform for protein detection. the presence of thrombin can induce the aptamer-modified fluorescent c-dots to form a sandwich structure with aptamer-functionalized silica nanoparticles through specific protein/aptamer interaction. the assay shows high specificity toward thrombin. a detection limit of 1 nm is obtained, which is significantly improved as compared to that of many previously reported fluorescence-based thrombin detection assays. using other modified aptamers and antibodies instead of thrombin binding aptamers, this strategy may offer a suitable approach for detection of other proteins in biological, pharmaceutical and nano-mechanical applications.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000310375200002
公开日期	2013-06-09
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48433]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Xu BL,Zhao CQ,Wei WL,et al. Aptamer carbon nanodot sandwich used for fluorescent detection of protein[J]. analyst,2012,137(23):5483-5486.
APA	Xu BL.,Zhao CQ.,Wei WL.,Ren JS.,Miyoshi D.,...&Qu XG.(2012).Aptamer carbon nanodot sandwich used for fluorescent detection of protein.analyst,137(23),5483-5486.
MLA	Xu BL,et al."Aptamer carbon nanodot sandwich used for fluorescent detection of protein".analyst 137.23(2012):5483-5486.