Adsorption Orientation of Horse Heart Cytochrome c on a Bare Gold Electrode Hampers Its Electron Transfer

	Adsorption Orientation of Horse Heart Cytochrome c on a Bare Gold Electrode Hampers Its Electron Transfer
	Lin SR ; Jiang XE ; Wang LX ; Li GH ; Guo LP
刊名	journal of physical chemistry c
	2012
卷号	116 期号:1 页码:637-642
关键词	SELF-ASSEMBLED MONOLAYERS INFRARED-ABSORPTION SPECTROSCOPY PROTEIN ADSORPTION STRUCTURAL-CHANGES MECHANISM SURFACES SERS
ISSN号	1932-7447
通讯作者	jiang xe
中文摘要	direct electron transfer of horse heart cytochrome c (hhcc) adsorbed on a bare metal electrode has been shown to be hard to achieve. the reason for that has been discussed in terms of conformational changes, protein unfolding, and even denaturation of the protein. we explored the adsorption of hhcc on a bare au electrode by surface-enhanced infrared absorption spectroscopy on the molecular level. our results revealed that the native secondary structure of adsorbed hhcc was kept even on the bare au surface. the hampered electron transfer was mainly caused by its adsorption orientation. the adsorption of hhcc on the bare gold surface led the tyrosine 97 residue to be close to the surface. in this orientation, most of the a-helices of adsorbed hhcc run parallel to the bare au surface with a flatter orientation of the heme relative to its orientation on an 11-mercaptoundecanoic acid-modified au surface.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000298978700079
公开日期	2013-06-09
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48395]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Lin SR,Jiang XE,Wang LX,et al. Adsorption Orientation of Horse Heart Cytochrome c on a Bare Gold Electrode Hampers Its Electron Transfer[J]. journal of physical chemistry c,2012,116(1):637-642.
APA	Lin SR,Jiang XE,Wang LX,Li GH,&Guo LP.(2012).Adsorption Orientation of Horse Heart Cytochrome c on a Bare Gold Electrode Hampers Its Electron Transfer.journal of physical chemistry c,116(1),637-642.
MLA	Lin SR,et al."Adsorption Orientation of Horse Heart Cytochrome c on a Bare Gold Electrode Hampers Its Electron Transfer".journal of physical chemistry c 116.1(2012):637-642.