Activity-regulated Somatostatin Expression Reduces Dendritic Spine Density and Lowers Excitatory Synaptic Transmission via Postsynaptic Somatostatin Receptor 4

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	Activity-regulated Somatostatin Expression Reduces Dendritic Spine Density and Lowers Excitatory Synaptic Transmission via Postsynaptic Somatostatin Receptor 4
	Hou, ZH ; Yu, X
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2013
卷号	288 期号:4 页码:2501-2509
关键词	CENTRAL-NERVOUS-SYSTEM RAT HIPPOCAMPUS DISYNAPTIC INHIBITION CORTICAL INTERNEURONS SOMATOSENSORY CORTEX NEURONAL DEVELOPMENT FLUORESCENT PROTEIN ELECTRICAL-ACTIVITY GABAERGIC NEURONS KAINIC ACID
ISSN号	0021-9258
通讯作者	Yu, X (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,yuxiang@ion.ac.cn
英文摘要	Neuronal activity regulates multiple aspects of the morphological and functional development of neural circuits. One mechanism by which it achieves this is through regulation of gene expression. In a screen for activity-induced genes, we identified somatostatin (SST), a neuropeptide secreted by the SST subtype of interneurons. Using real time quantitative PCR and ELISA, we showed that persistent elevation of neuronal activity increased both the gene expression and protein secretion of SST over a relatively prolonged time course of 48 h. Using primary hippocampal neuronal cultures, we found that SST treatment for 1 day significantly reduced the density of dendritic spines, the morphological bases of excitatory synapses. Furthermore, the density of pre- and postsynaptic markers of excitatory synapses was significantly lowered following SST treatment, whereas that of inhibitory synapses was not affected. Consistently, SST treatment reduced the frequency of miniature excitatory postsynaptic currents, without affecting inhibition. Finally, lowering the endogenous level of SST receptor subtype 4 in individual hippocampal pyramidal neurons significantly blocked the effect of SST in reducing spine density and excitatory synaptic transmission in a cell autonomous fashion, suggesting that the effect of SST in regulating excitatory synaptic transmission is mainly mediated by SST receptor subtype 4. Together, our results demonstrated that activity-dependent release of SST reduced the density of dendritic spines and the number of excitatory synapses through postsynaptic activation of SST receptor subtype 4 in pyramidal neurons. To our knowledge, this is the first demonstration of the long term effect of SST on neuronal morphology.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
资助信息	Ministry of Science and Technology [2011CBA00400]; National Natural Science Foundation of China [31125015, 31021063]
语种	英语
公开日期	2013-06-04
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2513]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Hou, ZH,Yu, X. Activity-regulated Somatostatin Expression Reduces Dendritic Spine Density and Lowers Excitatory Synaptic Transmission via Postsynaptic Somatostatin Receptor 4[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2013,288(4):2501-2509.
APA	Hou, ZH,&Yu, X.(2013).Activity-regulated Somatostatin Expression Reduces Dendritic Spine Density and Lowers Excitatory Synaptic Transmission via Postsynaptic Somatostatin Receptor 4.JOURNAL OF BIOLOGICAL CHEMISTRY,288(4),2501-2509.
MLA	Hou, ZH,et al."Activity-regulated Somatostatin Expression Reduces Dendritic Spine Density and Lowers Excitatory Synaptic Transmission via Postsynaptic Somatostatin Receptor 4".JOURNAL OF BIOLOGICAL CHEMISTRY 288.4(2013):2501-2509.