Thrombin Receptor Regulates Hematopoiesis and Endothelial-to-Hematopoietic Transition

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	Thrombin Receptor Regulates Hematopoiesis and Endothelial-to-Hematopoietic Transition
	Du, JL
刊名	DEVELOPMENTAL CELL
	2012
卷号	22 期号:5 页码:1092-1100
关键词	PROTEASE-ACTIVATED RECEPTORS EPITHELIAL-CELL PLASTICITY STEM-CELLS DEFINITIVE HEMATOPOIESIS EMBRYONIC-DEVELOPMENT AORTIC ENDOTHELIUM COUPLED RECEPTORS HAEMOGENIC ENDOTHELIUM ZEBRAFISH EMBRYO RHO-GTPASES
ISSN号	1534-5807
通讯作者	Pei, G (reprint author), Chinese Acad Sci, State Key Lab Cell Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci,Grad Sch, Shanghai 200031, Peoples R China.,gpei@sibs.ac.cn
英文摘要	Hematopoietic development and vascular development are closely related physiological processes during vertebrate embryogenesis. Recently, endothelial-to-hematopoietic transition (EHT) was demonstrated to be critical for hematopoietic stem and progenitor cell induction, but its underlying regulatory mechanisms remain poorly understood. Here we show that thrombin receptor (F2r), a protease-activated G protein-coupled receptor required for vascular development, functions as a negative regulator during hematopoietic development. F2r is significantly upregulated during hematopoietic differentiation of mouse embryonic stem cells (mESCs) and zebrafish hematopoietic development. Pharmacological or genetic inhibition of F2r promotes hematopoietic differentiation, whereas F2r overexpression shows opposite effects. Further mechanistic studies reveal that F2r-RhoA/ROCK pathway inhibits EHT in vitro and negatively regulates zebrafish EHT and hematopoietic stem cell induction in vivo. Taken together, this study demonstrates a fundamental role of F2r-RhoA/ROCK pathway in vertebrate hematopoiesis and EHT, as well as an important molecular mechanism coordinating hematopoietic and vascular development.
学科主题	Cell Biology ; Developmental Biology
收录类别	SCI
资助信息	Ministry of Science and Technology [2011CB946102, 2011CB910202, 2009CB940903, 2009CB941101, 2007CB947904, 2007CB948003, 2006CB943901, 2005CB522406]; Natural Science Foundation of Shanghai [10ZR1435300]; Chinese Academy of Sciences [SIBS2008001, KSCX2-YW-R-56, XDA01010302]; National Natural Science Foundation of China [90713047, 30621091]
语种	英语
公开日期	2013-06-04
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2505]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Du, JL. Thrombin Receptor Regulates Hematopoiesis and Endothelial-to-Hematopoietic Transition[J]. DEVELOPMENTAL CELL,2012,22(5):1092-1100.
APA	Du, JL.(2012).Thrombin Receptor Regulates Hematopoiesis and Endothelial-to-Hematopoietic Transition.DEVELOPMENTAL CELL,22(5),1092-1100.
MLA	Du, JL."Thrombin Receptor Regulates Hematopoiesis and Endothelial-to-Hematopoietic Transition".DEVELOPMENTAL CELL 22.5(2012):1092-1100.