DNA electrochemical biosensor based on thionine-graphene nanocomposite

	DNA electrochemical biosensor based on thionine-graphene nanocomposite
	Zhu LM ; Luo LQ ; Wang ZX
刊名	biosensors & bioelectronics
	2012
卷号	35 期号:1 页码:507-511
关键词	MODIFIED ELECTRODE CARBON NANOTUBES FILMS HYBRIDIZATION OXIDE SENSORS SHEETS WATER GENE
ISSN号	0956-5663
通讯作者	luo lq
中文摘要	a novel protocol for development of dna electrochemical biosensor based on thionine-graphene nanocomposite modified gold electrode was presented. the thionine-graphene nanocomposite layer with highly conductive property was characterized by scanning electron microscopy, transmission electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. an amino-substituted oligonucleotide probe was covalently grafted onto the surface of the thionine-graphene nanocomposite by the cross-linker glutaraldehyde. the hybridization reaction on the modified electrode was monitored by differential pulse voltammetry analysis using an electroactive intercalator daunomycin as the indicator. under optimum conditions, the proposed biosensor exhibited high sensitivity and low detection limit for detecting complementary oligonucleotide. the complementary oligonucleotide could be quantified in a wide range of 1.0 x 10(-12) to 1.0 x 10(-7) m with a good linearity (r-2 = 0.9976) and a low detection limit of 1.26 x 10(-13) m (s/n = 3). in addition, the biosensor was highly selective to discriminate one-base or two-base mismatched sequences. (c) 2012 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000305036000083
公开日期	2013-05-20
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48162]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zhu LM,Luo LQ,Wang ZX. DNA electrochemical biosensor based on thionine-graphene nanocomposite[J]. biosensors & bioelectronics,2012,35(1):507-511.
APA	Zhu LM,Luo LQ,&Wang ZX.(2012).DNA electrochemical biosensor based on thionine-graphene nanocomposite.biosensors & bioelectronics,35(1),507-511.
MLA	Zhu LM,et al."DNA electrochemical biosensor based on thionine-graphene nanocomposite".biosensors & bioelectronics 35.1(2012):507-511.