Multidomain Protein Solves the Folding Problem by Multifunnel Combined Landscape: Theoretical Investigation of a Y-Family DNA Polymerase

	Multidomain Protein Solves the Folding Problem by Multifunnel Combined Landscape: Theoretical Investigation of a Y-Family DNA Polymerase
	Wang Y ; Chu XK ; Suo ZC ; Wang EK ; Wang J
刊名	journal of the american chemical society
	2012
卷号	134 期号:33 页码:13755-13764
关键词	MOLECULAR-DYNAMICS SIMULATIONS MONOMERIC LACTOSE REPRESSOR LARGE 2-DOMAIN PROTEIN ENERGY LANDSCAPE ADENYLATE-KINASE CONFORMATIONAL TRANSITIONS LEVINTHAL PARADOX BYPASS PATHWAYS COOPERATIVITY
ISSN号	0002-7863
通讯作者	wang j
中文摘要	approximately three-fourths of eukaryotic proteins are composed of multiple independently folded domains. however, much of our understanding is based on single domain proteins or isolated domains whose studies directly lead to well-known energy landscape theory in which proteins fold by navigating through a funneled energy landscape toward native structure ensembles. the degrees of freedom for proteins with multiple domains are many orders of magnitude larger than that for single domain proteins. now, the question arises: how do the multidomain proteins solve the "protein folding problem"? here, we specifically address this issue by exploring the structure folding relationship of sulfolobus solfataricus dna polymerase iv (dpo4), a prototype y-family dna polymerase which contains a polymerase core consisting of a palm (p domain), a finger (f domain), and a thumb domain (t domain) in addition to a little finger domain (lf domain). the theoretical results are in good agreement with the experimental data and lead to several theoretical predictions. finally, we propose that for rapid folding into well-defined conformations which carry out the biological functions, four domain dpo4 employs a divide-and-conquer strategy, that is, combining multiple individual folding funnels into a single funnel (domains fold independently and then coalesce). in this way,. the degrees of freedom for multidomain proteins are polynomial rather than exponential, and the conformational search process can be reduced effectively from a large to a smaller time scale.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000307699000035
公开日期	2013-04-23
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/47994]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang Y,Chu XK,Suo ZC,et al. Multidomain Protein Solves the Folding Problem by Multifunnel Combined Landscape: Theoretical Investigation of a Y-Family DNA Polymerase[J]. journal of the american chemical society,2012,134(33):13755-13764.
APA	Wang Y,Chu XK,Suo ZC,Wang EK,&Wang J.(2012).Multidomain Protein Solves the Folding Problem by Multifunnel Combined Landscape: Theoretical Investigation of a Y-Family DNA Polymerase.journal of the american chemical society,134(33),13755-13764.
MLA	Wang Y,et al."Multidomain Protein Solves the Folding Problem by Multifunnel Combined Landscape: Theoretical Investigation of a Y-Family DNA Polymerase".journal of the american chemical society 134.33(2012):13755-13764.