Graphene Enhanced Electron Transfer at Aptamer Modified Electrode and Its Application in Biosensing

	Graphene Enhanced Electron Transfer at Aptamer Modified Electrode and Its Application in Biosensing
	Wang L ; Xu M ; Han L ; Zhou M ; Zhu CZ ; Dong SJ
刊名	analytical chemistry
	2012
卷号	84 期号:17 页码:7301-7307
关键词	DNA SELECTION CARBON OXIDE ARCHITECTURE MOLECULES PLATFORM LIGANDS SENSORS INVITRO
ISSN号	0003-2700
通讯作者	dong sj
中文摘要	graphene (gn), a two-dimensional and one-atom thick carbon sheet, is showing exciting applications because of its unique morphology and properties. in this work, a new electrochemical biosensing platform by taking advantage of the ultrahigh electron transfer ability of gn and its unique gn/ssdna interaction was reported. adenosine triphosphate binding aptamer (aba) immobilized on au electrode could strongly adsorb gn due to the strong pi-pi interaction and resulted in a large decrease of the charge transfer resistance (r-ct) of the electrode. however, the binding reaction between aba and its target adenosine triphosphate (atp) inhibited the adsorption of gn, and r-ct could not be decreased. on the basis of this, we developed a new gn-based biosensing platform for the detection of small molecule atp. the experimental results confirmed that the electrochemical aptasensor we developed possessed a good sensitivity and high selectivity for atp. the detection range for atp was from 15 x 10(-9) to 4 x 10(-3) m. the method here was label-free and sensitive and did not require sophisticated fabrication. furthermore, we can generalize this strategy to detect hg2+ using a thymine (t)-rich, mercury-specific oligonucleotide. therefore, we expected that this method may offer a promising approach for designing high-performance electrochemical aptasensors for the sensitive and selective detection of a spectrum of targets.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000308255400006
公开日期	2013-04-23
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/47954]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang L,Xu M,Han L,et al. Graphene Enhanced Electron Transfer at Aptamer Modified Electrode and Its Application in Biosensing[J]. analytical chemistry,2012,84(17):7301-7307.
APA	Wang L,Xu M,Han L,Zhou M,Zhu CZ,&Dong SJ.(2012).Graphene Enhanced Electron Transfer at Aptamer Modified Electrode and Its Application in Biosensing.analytical chemistry,84(17),7301-7307.
MLA	Wang L,et al."Graphene Enhanced Electron Transfer at Aptamer Modified Electrode and Its Application in Biosensing".analytical chemistry 84.17(2012):7301-7307.