Heterotrophic cultivation of Chlorella kessleri for fatty acids production by carbon and nitrogen supplements

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Heterotrophic cultivation of Chlorella kessleri for fatty acids production by carbon and nitrogen supplements
	Wang, Yan1 ; Chen, Tao 2; Qin, Song1
刊名	BIOMASS & BIOENERGY
	2012-12-01
卷号	47 页码:402-409
关键词	Chlorella kessleri Fatty acid Nitrogen-starvation Heterotrophic
ISSN号	0961-9534
通讯作者	Wang, Y (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China.,ywang@yic.ac.cn
产权排序	[Wang, Yan; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Chen, Tao] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China
中文摘要	Green microalga Chlorella kessleri was grown in the dark with glucose (100 mmol l(-1)) and nitrate (10 mmol l(-1)) as initial carbon and nitrogen sources, respectively. During the cultivation, extra exogenous glucose (100 mmol l(-1)) and nitrate (10 mmol l(-1)) were singly or simultaneously supplied at interval through different strategies. Results showed that under similar concentration and frequency of glucose recharge, suitable reduction in the frequency of nitrate addition can make cells accumulate more fatty acids, while the dry biomass was still maintained at a high level. As a result, the highest biomass concentration (17.60 g l(-1)), total fatty acids content (47.67% of dry weight), and total fatty acids yield (8.39 g l(-1)) could be simultaneously obtained in the same culture, designated as intermittent C-N-feeding culture. On the other hand, since that recharge of other substrates such as fresh medium is not needed, the present intermittent C-N-feeding process is relatively easy-control and low-cost. (C) 2012 Elsevier Ltd. All rights reserved.
英文摘要	Green microalga Chlorella kessleri was grown in the dark with glucose (100 mmol l(-1)) and nitrate (10 mmol l(-1)) as initial carbon and nitrogen sources, respectively. During the cultivation, extra exogenous glucose (100 mmol l(-1)) and nitrate (10 mmol l(-1)) were singly or simultaneously supplied at interval through different strategies. Results showed that under similar concentration and frequency of glucose recharge, suitable reduction in the frequency of nitrate addition can make cells accumulate more fatty acids, while the dry biomass was still maintained at a high level. As a result, the highest biomass concentration (17.60 g l(-1)), total fatty acids content (47.67% of dry weight), and total fatty acids yield (8.39 g l(-1)) could be simultaneously obtained in the same culture, designated as intermittent C-N-feeding culture. On the other hand, since that recharge of other substrates such as fresh medium is not needed, the present intermittent C-N-feeding process is relatively easy-control and low-cost. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	LIPID-ACCUMULATION ; WASTE-WATER ; MICROALGAE ; BIODIESEL ; GROWTH ; BATCH ; VULGARIS ; OIL ; MICROORGANISMS ; STARVATION
收录类别	SCI
资助信息	National Natural Science Foundation of China [50904051]; Science and Technology Planning Project of Yantai, China [2010247]; Shandong Oriental Ocean Sci-Tech Co., Ltd [200803]
原文出处	http://dx.doi.org/10.1016/j.biombioe.2012.09.018
语种	英语
WOS记录号	WOS:000314075900043
公开日期	2013-03-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6115]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yan,Chen, Tao,Qin, Song. Heterotrophic cultivation of Chlorella kessleri for fatty acids production by carbon and nitrogen supplements[J]. BIOMASS & BIOENERGY,2012,47:402-409.
APA	Wang, Yan,Chen, Tao,&Qin, Song.(2012).Heterotrophic cultivation of Chlorella kessleri for fatty acids production by carbon and nitrogen supplements.BIOMASS & BIOENERGY,47,402-409.
MLA	Wang, Yan,et al."Heterotrophic cultivation of Chlorella kessleri for fatty acids production by carbon and nitrogen supplements".BIOMASS & BIOENERGY 47(2012):402-409.