Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis
	Wen, JJ ; Xie, J ; Liu, SG ; Gui, JF
刊名	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
	2001-10-01
卷号	44 期号:5 页码:541-553
关键词	silver crucian carp oocyte oogenesis suppressive subtractive hybridization WD domain
ISSN号	1006-9305
通讯作者	Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	A new gene with WD domains is cloned and characterized according to its differential transcription and expression between previtellogenic oocytes (phase I oocytes) and fully-grown oocytes (phase V oocytes) from natural gynogenetic silver crucian carp (Carassius auratus gibelio) by using the combinative methods of suppressive subtraction hybridization, SMART cDNA synthesis and RACE-PCR. The full-length cDNA is 1870 bp. Its 5 ' untranslated region is 210 bp, followed by an open reading frame of 990 bp, which has the typical vertebrate initiator codon of ANNATG. The open reading frame encodes a protein with 329 amino acids. It has 670 bp of 3 ' untranslated region and an AATAAA polyadenylation signal. Because it has 92% homology to STRAP (serine-threonine kinase receptor-associated protein), a recently reported gene, we named it FSTRAP (fish STRAP). Virtual Northern blotting indicated that the FSTRAP was transcribed in fully-grown oocytes (phase V oocytes), but not in previtellogenic oocytes (phase I oocytes). RT-PCR analysis showed that FSTRAP was transcribed in brain, heart, kidney, muscle, ovary, spleen and testis, but not in liver. And its mRNA could be detected in the oocytes from phase II to phase V. Western blotting also showed that FSTRAP protein could be detected in brain, heart, kidney, muscle, ovary, spleen and testis except liver. Results of Western blotting on various oocytes were also similar to the RT-PCR data. FSTRAP protein was not expressed in the previtellogenic oocytes. Its expression initiated from phase II oocytes after vitellogenesis, and was consistent with the mRNA transcription.
英文摘要	A new gene with WD domains is cloned and characterized according to its differential transcription and expression between previtellogenic oocytes (phase I oocytes) and fully-grown oocytes (phase V oocytes) from natural gynogenetic silver crucian carp (Carassius auratus gibelio) by using the combinative methods of suppressive subtraction hybridization, SMART cDNA synthesis and RACE-PCR. The full-length cDNA is 1870 bp. Its 5 ' untranslated region is 210 bp, followed by an open reading frame of 990 bp, which has the typical vertebrate initiator codon of ANNATG. The open reading frame encodes a protein with 329 amino acids. It has 670 bp of 3 ' untranslated region and an AATAAA polyadenylation signal. Because it has 92% homology to STRAP (serine-threonine kinase receptor-associated protein), a recently reported gene, we named it FSTRAP (fish STRAP). Virtual Northern blotting indicated that the FSTRAP was transcribed in fully-grown oocytes (phase V oocytes), but not in previtellogenic oocytes (phase I oocytes). RT-PCR analysis showed that FSTRAP was transcribed in brain, heart, kidney, muscle, ovary, spleen and testis, but not in liver. And its mRNA could be detected in the oocytes from phase II to phase V. Western blotting also showed that FSTRAP protein could be detected in brain, heart, kidney, muscle, ovary, spleen and testis except liver. Results of Western blotting on various oocytes were also similar to the RT-PCR data. FSTRAP protein was not expressed in the previtellogenic oocytes. Its expression initiated from phase II oocytes after vitellogenesis, and was consistent with the mRNA transcription.
学科主题	Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biology
研究领域[WOS]	Life Sciences & Biomedicine - Other Topics
关键词[WOS]	SILVER CRUCIAN CARP ; GROWTH-FACTOR-BETA ; TROUT ONCORHYNCHUS-MYKISS ; XENOPUS-LAEVIS OOCYTES ; AURATUS GIBELIO BLOCH ; I IGF-I ; RAINBOW-TROUT ; INVOLVEMENT ; SUPPRESSION ; RECEPTORS
收录类别	SCI
语种	英语
WOS记录号	WOS:000171966600011
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9920]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China
推荐引用方式 GB/T 7714	Wen, JJ,Xie, J,Liu, SG,et al. Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2001,44(5):541-553.
APA	Wen, JJ,Xie, J,Liu, SG,&Gui, JF.(2001).Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,44(5),541-553.
MLA	Wen, JJ,et al."Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 44.5(2001):541-553.