Effects of cell-cycle arrest agents on cleavage and development of loach (Misgurnus anguillicaudatus) embryos

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	Effects of cell-cycle arrest agents on cleavage and development of loach (Misgurnus anguillicaudatus) embryos
	Liu, TM; Wu, CJ; Yu, XM; Li, XY
刊名	FISH PHYSIOLOGY AND BIOCHEMISTRY
	2001
卷号	25 期号:1 页码:3-9
关键词	cell cycle arrest colchicine loach nocodazole reversibility synchronization toxicity
ISSN号	0920-1742
通讯作者	Wu, CJ, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
中文摘要	The aim of this study was to determine the lowest concentration of nocodazole and colchicine to arrest blastomere division during the cleavage stage of loach embryos and to assess the reversibility and toxicity of the treatments in the treated embryos. Eight-cell loach embryos were incubated for 4, 8, 12, or 16 h in 1/10x Holtfreter supplemented with either nocodazole, an inhibitor of tubulin polymerization, or colchicine, an inhibitor of tubulin assembly. Complete arrest of cell cycle was observed, at a colchicine concentration of 0.996 mM and at a nocodazole concentration of 0.275 muM, respectively (the lowest effective concentration). No major morphological alteration in chromatin was observed. Reversibility and toxicity of both agents were dose and exposure period dependent. For both agents, prolonging cleavage arrest for more than 4 h (at the effective concentrations) is detrimental to development of embryos. Nocodazole treatment was less cytotoxic, whereas the concentrations of colchicine which induce cleavage arrest were detrimental to development beyond the blastula stage. Toxic effects beyond the blastula stage could be minimized for both agents by reducing the period of treatment and concentration.
英文摘要	The aim of this study was to determine the lowest concentration of nocodazole and colchicine to arrest blastomere division during the cleavage stage of loach embryos and to assess the reversibility and toxicity of the treatments in the treated embryos. Eight-cell loach embryos were incubated for 4, 8, 12, or 16 h in 1/10x Holtfreter supplemented with either nocodazole, an inhibitor of tubulin polymerization, or colchicine, an inhibitor of tubulin assembly. Complete arrest of cell cycle was observed, at a colchicine concentration of 0.996 mM and at a nocodazole concentration of 0.275 muM, respectively (the lowest effective concentration). No major morphological alteration in chromatin was observed. Reversibility and toxicity of both agents were dose and exposure period dependent. For both agents, prolonging cleavage arrest for more than 4 h (at the effective concentrations) is detrimental to development of embryos. Nocodazole treatment was less cytotoxic, whereas the concentrations of colchicine which induce cleavage arrest were detrimental to development beyond the blastula stage. Toxic effects beyond the blastula stage could be minimized for both agents by reducing the period of treatment and concentration.
学科主题	Biochemistry & Molecular Biology; Fisheries; Physiology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Fisheries ; Physiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Fisheries ; Physiology
关键词[WOS]	NUCLEAR TRANSFER EMBRYOS ; MOUSE EMBRYOS ; BOVINE EMBRYOS ; ENUCLEATED OOCYTES ; IN-VITRO ; DIVISION ; TRANSPLANTATION ; SYNCHRONIZATION ; BLASTOMERES ; NOCODAZOLE
收录类别	SCI
语种	英语
WOS记录号	WOS:000177379800001
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9818]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Liu, TM,Wu, CJ,Yu, XM,et al. Effects of cell-cycle arrest agents on cleavage and development of loach (Misgurnus anguillicaudatus) embryos[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2001,25(1):3-9.
APA	Liu, TM,Wu, CJ,Yu, XM,&Li, XY.(2001).Effects of cell-cycle arrest agents on cleavage and development of loach (Misgurnus anguillicaudatus) embryos.FISH PHYSIOLOGY AND BIOCHEMISTRY,25(1),3-9.
MLA	Liu, TM,et al."Effects of cell-cycle arrest agents on cleavage and development of loach (Misgurnus anguillicaudatus) embryos".FISH PHYSIOLOGY AND BIOCHEMISTRY 25.1(2001):3-9.