Multilayer hydroxyethyl cellulose-adsorbed coating on poly(dimethylsiloxane) microchips for protein separation

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	Multilayer hydroxyethyl cellulose-adsorbed coating on poly(dimethylsiloxane) microchips for protein separation
	Liu CY(刘春叶) ; Xu X(许旭) ; Chen JR(陈杰瑢)
刊名	Can. J. Anal. Sci. Spectrosc.
	2008
卷号	53 期号:4 页码:171-178
ISSN号	1205-6685
其他题名	Multilayer hydroxyethyl cellulose-adsorbed coating on poly(dimethylsiloxane) microchips for protein separation
英文摘要	In this paper, we present a fast method for generation of hydrophilic surface coating for poly(dimethylsiloxane) (PDMS) microchip. The native PDMS surface was modified by multilayer-adsorbed and heat-immobilized hydroxyethylcellulose (HEC) after hydrogen peroxide solution treatment. Repeating the coating procedure three times was found to produce the most effective coating. The three-layer HEC coating converts the original PDMS surface from a hydrophobic one into a hydrophilic surface, and suppressed the electroosmotic flow (EOF) in HEC-coated channels over a wide pH range of 3-11. Four fluorescently labeled enzymes derived from multienzyme tablet were separated satisfactorily on the HEC-adsorbed PDMS microchip. Good separation of standard proteins based on molecular weight was also obtained on the coated chip.
学科主题	分析化学
收录类别	SCI
语种	英语
WOS记录号	WOS:000267201200003
公开日期	2013-03-12
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/24769]
专题	上海有机化学研究所_上海有机化学研究所
推荐引用方式 GB/T 7714	Liu CY,Xu X,Chen JR. Multilayer hydroxyethyl cellulose-adsorbed coating on poly(dimethylsiloxane) microchips for protein separation[J]. Can. J. Anal. Sci. Spectrosc.,2008,53(4):171-178.
APA	刘春叶,许旭,&陈杰瑢.(2008).Multilayer hydroxyethyl cellulose-adsorbed coating on poly(dimethylsiloxane) microchips for protein separation.Can. J. Anal. Sci. Spectrosc.,53(4),171-178.
MLA	刘春叶,et al."Multilayer hydroxyethyl cellulose-adsorbed coating on poly(dimethylsiloxane) microchips for protein separation".Can. J. Anal. Sci. Spectrosc. 53.4(2008):171-178.