Infection and propagation of lymphocystis virus isolated from the cultured flounder Paralichthys olivaceus in grass carp cell lines

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Infection and propagation of lymphocystis virus isolated from the cultured flounder Paralichthys olivaceus in grass carp cell lines
	Zhang, QY; Ruan, HM; Li, ZQ; Yuan, XP; Gui, HF
刊名	DISEASES OF AQUATIC ORGANISMS
	2003-12-03
卷号	57 期号:1-2 页码:27-34
关键词	lymphocystis virus LV iridovirus viral disease freshwater fish cell line apoptosis
ISSN号	0177-5103
通讯作者	Gui, HF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	The causative agent of lymphocystis disease that frequently occurs in cultured flounder Paralichthys olivaceus in China is lymphocystis virus (LV). In this study, 13 fish cell lines were tested for their susceptibility to LV. Of these, 2 cell lines derived from the freshwater grass carp Ctenopharyngodon idellus proved susceptible to the LV, and 1 cell line, GCO (grass carp ovary), was therefore used to replicate and propagate the virus. An obvious cytopathic effect (CPE) was first observed in cell monolayers at 1 d post-inoculation, and at 3 d this had extended to about 75% of the cell monolayer. However, no further CPE extension was observed after 4 d. Cytopathic characteristics induced by the LV were detected by Giemsa staining and fluorescence microscopic observation with Hoechst 33258 staining. The propagated virus particles were also observed by electron microscopy. Ultrastructure analysis revealed several distinct cellular changes, such as chromatin compaction and margination, vesicle formation, cell-surface convolution, nuclear fragmentation and the occurrence of characteristic 'blebs' and cell fusion. This study provides a detailed report of LV infection and propagation in a freshwater fish cell line, and presents direct electron microscopy evidence for propagation of the virus in infected cells. A possible process by which the CPEs are controlled is suggested.
英文摘要	The causative agent of lymphocystis disease that frequently occurs in cultured flounder Paralichthys olivaceus in China is lymphocystis virus (LV). In this study, 13 fish cell lines were tested for their susceptibility to LV. Of these, 2 cell lines derived from the freshwater grass carp Ctenopharyngodon idellus proved susceptible to the LV, and 1 cell line, GCO (grass carp ovary), was therefore used to replicate and propagate the virus. An obvious cytopathic effect (CPE) was first observed in cell monolayers at 1 d post-inoculation, and at 3 d this had extended to about 75% of the cell monolayer. However, no further CPE extension was observed after 4 d. Cytopathic characteristics induced by the LV were detected by Giemsa staining and fluorescence microscopic observation with Hoechst 33258 staining. The propagated virus particles were also observed by electron microscopy. Ultrastructure analysis revealed several distinct cellular changes, such as chromatin compaction and margination, vesicle formation, cell-surface convolution, nuclear fragmentation and the occurrence of characteristic 'blebs' and cell fusion. This study provides a detailed report of LV infection and propagation in a freshwater fish cell line, and presents direct electron microscopy evidence for propagation of the virus in infected cells. A possible process by which the CPEs are controlled is suggested.
学科主题	Fisheries; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Veterinary Sciences
关键词[WOS]	DISEASE VIRUS ; RANA-GRYLIO ; FISH ; ULTRASTRUCTURE ; PLEURONECTES ; IRIDOVIRUS ; TOXICITY ; PROTEIN ; TUMORS
收录类别	SCI
语种	英语
WOS记录号	WOS:000189277700004
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9546]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhang, QY,Ruan, HM,Li, ZQ,et al. Infection and propagation of lymphocystis virus isolated from the cultured flounder Paralichthys olivaceus in grass carp cell lines[J]. DISEASES OF AQUATIC ORGANISMS,2003,57(1-2):27-34.
APA	Zhang, QY,Ruan, HM,Li, ZQ,Yuan, XP,&Gui, HF.(2003).Infection and propagation of lymphocystis virus isolated from the cultured flounder Paralichthys olivaceus in grass carp cell lines.DISEASES OF AQUATIC ORGANISMS,57(1-2),27-34.
MLA	Zhang, QY,et al."Infection and propagation of lymphocystis virus isolated from the cultured flounder Paralichthys olivaceus in grass carp cell lines".DISEASES OF AQUATIC ORGANISMS 57.1-2(2003):27-34.