Preparation of pH-sensitive polymer by thermal initiation polymerization and its application in fluorescence immunoassay

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Preparation of pH-sensitive polymer by thermal initiation polymerization and its application in fluorescence immunoassay
	Lin, P ; Feng, JJ ; Zheng, H ; Yang, HH ; Xu, JG
刊名	TALANTA
	2005-01-30
卷号	65 期号:2 页码:430-436
关键词	pH-sensitive phase separating polymer fluorescence immunoassay human IgG
ISSN号	0039-9140
通讯作者	Xu, JG, Xiamen Univ, Dept Chem, MOE, Key Lab Analyt Sci, Xiamen 361005, Peoples R China
中文摘要	A fluorescence immunoassay for human IgG (Ag) was developed using a pH-sensitive polymer prepared by thermal initiation or redox initiation polymerization as a carrier. In the competitive immunoassay, appropriate quantity of Ag was immobilized on the polymer and the standard Ag (or sample) solution, and a constant amount of fluorescein isothiocyanate labeled goat anti-human IgG antibody (Ab-FITC) was added. Immobilized Ag and the standard (or sample) Ag competed for binding to the Ab-FITC in 37 C in homogeneous format. After changing the pH to separate the polymer-immune complex precipitate, it was re-dissolved and determined by fluorescence method. The results showed that the immobilization efficiency, immunological reaction activities of immobilized Au and phase transition pH range were improved as Ag was immobilized by thermal initiation instead of redox initiation polymerization. Under optimum conditions, the calibration graphs for the Ag in both methods, thermal initiation and redox initiation, were linear over the concentration range of 0.0-1000 ng mL(-1), with detection limits 8 (thermal initiation) and 12 ng mL(1) (redox initiation), respectively. Moreover, some pH-sensitive polymer prepared only in organic solvent or under high temperature could also be used as an immunoreaction carrier by thermal initiation polymerization. Thermal initiation polymerization was a better immobilization mode. (C) 2004 Elsevier B.V. All rights reserved.
英文摘要	A fluorescence immunoassay for human IgG (Ag) was developed using a pH-sensitive polymer prepared by thermal initiation or redox initiation polymerization as a carrier. In the competitive immunoassay, appropriate quantity of Ag was immobilized on the polymer and the standard Ag (or sample) solution, and a constant amount of fluorescein isothiocyanate labeled goat anti-human IgG antibody (Ab-FITC) was added. Immobilized Ag and the standard (or sample) Ag competed for binding to the Ab-FITC in 37 C in homogeneous format. After changing the pH to separate the polymer-immune complex precipitate, it was re-dissolved and determined by fluorescence method. The results showed that the immobilization efficiency, immunological reaction activities of immobilized Au and phase transition pH range were improved as Ag was immobilized by thermal initiation instead of redox initiation polymerization. Under optimum conditions, the calibration graphs for the Ag in both methods, thermal initiation and redox initiation, were linear over the concentration range of 0.0-1000 ng mL(-1), with detection limits 8 (thermal initiation) and 12 ng mL(1) (redox initiation), respectively. Moreover, some pH-sensitive polymer prepared only in organic solvent or under high temperature could also be used as an immunoreaction carrier by thermal initiation polymerization. Thermal initiation polymerization was a better immobilization mode. (C) 2004 Elsevier B.V. All rights reserved.
学科主题	Chemistry ; Analytical
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	PHASE-SEPARATING POLYMER ; TIME-RESOLVED FLUOROIMMUNOASSAY ; HYDROGELS ; SYSTEM ; PROTEIN ; IMMOBILIZATION ; DELIVERY ; POINT ; ACID)
收录类别	SCI
语种	英语
WOS记录号	WOS:000225381900019
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9370]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Xiamen Univ, Dept Chem, MOE, Key Lab Analyt Sci, Xiamen 361005, Peoples R China 2.Jimei Univ, Coll Fisheries, Xiamen 361021, Peoples R China 3.Jimei Univ, Inst Aquaculture Biotechnol, Xiamen 361021, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Lin, P,Feng, JJ,Zheng, H,et al. Preparation of pH-sensitive polymer by thermal initiation polymerization and its application in fluorescence immunoassay[J]. TALANTA,2005,65(2):430-436.
APA	Lin, P,Feng, JJ,Zheng, H,Yang, HH,&Xu, JG.(2005).Preparation of pH-sensitive polymer by thermal initiation polymerization and its application in fluorescence immunoassay.TALANTA,65(2),430-436.
MLA	Lin, P,et al."Preparation of pH-sensitive polymer by thermal initiation polymerization and its application in fluorescence immunoassay".TALANTA 65.2(2005):430-436.