Electron microscopic examination of the viromatrix of Rana grylio virus in a fish cell line

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	Electron microscopic examination of the viromatrix of Rana grylio virus in a fish cell line
	Huang, XH ; Huang, YH ; Yuan, XP ; Zhang, QY
刊名	JOURNAL OF VIROLOGICAL METHODS
	2006-05-01
卷号	133 期号:2 页码:117-123
关键词	iridovirus RGV viromatrix ultrastructure quantitative analysis
ISSN号	0166-0934
通讯作者	Zhang, QY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	Rana grylio virus (RGV), a Ranavirus belonging to the family Iridoviridae, assembles in the viromatrix which is a factory for viral genome replication and particle assembly. Ultrastructural studies of the viromatrix will clarify the pathway of assembly. The viromatrix and quantitative changes in RGV infected epithelipma papulosum cyprini (EPC) cells, one of fish cell lines, were studied by electron microscopy. It was shown that viromatrices were adjacent to the nucleus, and the electron density was lower than that of the surrounding cytoplasm. The viromatrix contained virus particles with different forms, electron-dense materials and amorphous structures which included tubules and membranous materials. Tubules were often observed in direct continuity with empty capsids. Several bundles of intermediate filaments were seen alongside the viromatrix and crystalline aggregates. Large clusters of mitochondria occurred in proximity to viromatrix. A total of 990 cells profiles were examined. The results showed that 394 cells contained viromatrix: 89.3% contained one, and 10.7% contained two to four viromatrices. The number of viromatrices increased gradually and reached a peak at 16 h p.i. The viromatrix area at 24 h p.i. increased up to 7.4 +/- 0.69 mu m(2) which was three-times lower than that at 6 h p.i. The number of empty capsids within viromatrix was generally more than that of "full" particles at different time points, and there was a strong positive correlation between them. (c) 2005 Elsevier B.V. All rights reserved.
英文摘要	Rana grylio virus (RGV), a Ranavirus belonging to the family Iridoviridae, assembles in the viromatrix which is a factory for viral genome replication and particle assembly. Ultrastructural studies of the viromatrix will clarify the pathway of assembly. The viromatrix and quantitative changes in RGV infected epithelipma papulosum cyprini (EPC) cells, one of fish cell lines, were studied by electron microscopy. It was shown that viromatrices were adjacent to the nucleus, and the electron density was lower than that of the surrounding cytoplasm. The viromatrix contained virus particles with different forms, electron-dense materials and amorphous structures which included tubules and membranous materials. Tubules were often observed in direct continuity with empty capsids. Several bundles of intermediate filaments were seen alongside the viromatrix and crystalline aggregates. Large clusters of mitochondria occurred in proximity to viromatrix. A total of 990 cells profiles were examined. The results showed that 394 cells contained viromatrix: 89.3% contained one, and 10.7% contained two to four viromatrices. The number of viromatrices increased gradually and reached a peak at 16 h p.i. The viromatrix area at 24 h p.i. increased up to 7.4 +/- 0.69 mu m(2) which was three-times lower than that at 6 h p.i. The number of empty capsids within viromatrix was generally more than that of "full" particles at different time points, and there was a strong positive correlation between them. (c) 2005 Elsevier B.V. All rights reserved.
学科主题	Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
关键词[WOS]	SWINE-FEVER VIRUS ; QUANTITATIVE ULTRASTRUCTURAL ANALYSIS ; VACCINIA VIRUS ; ENDOPLASMIC-RETICULUM ; INTERMEDIATE COMPARTMENT ; ASSEMBLY SITES ; INFECTED CELLS ; MICROTUBULES ; MORPHOGENESIS ; IRIDOVIRUS
收录类别	SCI
语种	英语
WOS记录号	WOS:000236956200001
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8990]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 10039, Peoples R China
推荐引用方式 GB/T 7714	Huang, XH,Huang, YH,Yuan, XP,et al. Electron microscopic examination of the viromatrix of Rana grylio virus in a fish cell line[J]. JOURNAL OF VIROLOGICAL METHODS,2006,133(2):117-123.
APA	Huang, XH,Huang, YH,Yuan, XP,&Zhang, QY.(2006).Electron microscopic examination of the viromatrix of Rana grylio virus in a fish cell line.JOURNAL OF VIROLOGICAL METHODS,133(2),117-123.
MLA	Huang, XH,et al."Electron microscopic examination of the viromatrix of Rana grylio virus in a fish cell line".JOURNAL OF VIROLOGICAL METHODS 133.2(2006):117-123.