Molecular cloning and stress-induced expression of paralichthys olivaceus heme-regulated initiation factor 2 alpha kinase

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	Molecular cloning and stress-induced expression of paralichthys olivaceus heme-regulated initiation factor 2 alpha kinase
	Zhu, Rong ; Zhang, Yi-Bing ; Chen, Yu-Dong ; Dong, Cai-Wen ; Zhang, Fu-Tie ; Zhang, Qi-Ya ; Gui, Jian-Fang
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2006
卷号	30 期号:11 页码:1047-1059
关键词	heme-regulated initiation factor 2 alpha kinase (HRI) grass carp haemorrhagic virus (GCHV) flounder (paralichthys olivaceus) embryonic cells (FEC) inductive expression stress responses
通讯作者	Gui, JF, Chinese Acad Sci, Grad Sch, Chinese Acad Sci, Inst Hydrobiol,State Key Lab Freshwater Ecol & Bi, Wuhan 430072, Peoples R China
中文摘要	The heme-regulated initiation factor 2 alpha kinase (HRI) is acknowledged to play an important role in translational shutoff in reticulocytes in response to various cellular stresses. In this study, we report its homologous cDNA cloning and characterization from cultured flounder embryonic cells (FEC) after treatment with UV-inactivated grass carp haemorrhagic virus (GCHV). The full-length cDNA of Paralichthys olivaceus HRI homologue (PoHRI) has 2391 bp and encodes a protein of 651 amino acids. The putative PoHRI protein exhibits high identity with all members of eIF2 alpha kinase family. It contains 12 catalytic subdomains located within the C-terminus of all Ser/Thr protein kinases, a unique kinase insertion of 136 amino acids between subdomains IV and V, and a relatively conserved N-terminal domain (NTD). Upon heat shock, virus infection or Poly PC treatment, PoHRI mRNA and protein are significantly upregulated in FEC cells but show different expression patterns in response to different stresses. In healthy flounders, PoHRI displays a wide tissue distribution at both the mRNA and protein levels. These results indicate that PoHRI is a ubiquitous eIF2a kinase and might play an important role in translational control over nonheme producing FEC cells under different stresses. (c) 2006 Elsevier Ltd. All rights reserved.
学科主题	Immunology; Zoology
收录类别	SCI
语种	英语
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8830]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
推荐引用方式 GB/T 7714	Zhu, Rong,Zhang, Yi-Bing,Chen, Yu-Dong,et al. Molecular cloning and stress-induced expression of paralichthys olivaceus heme-regulated initiation factor 2 alpha kinase[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2006,30(11):1047-1059.
APA	Zhu, Rong.,Zhang, Yi-Bing.,Chen, Yu-Dong.,Dong, Cai-Wen.,Zhang, Fu-Tie.,...&Gui, Jian-Fang.(2006).Molecular cloning and stress-induced expression of paralichthys olivaceus heme-regulated initiation factor 2 alpha kinase.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,30(11),1047-1059.
MLA	Zhu, Rong,et al."Molecular cloning and stress-induced expression of paralichthys olivaceus heme-regulated initiation factor 2 alpha kinase".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 30.11(2006):1047-1059.