Morphological and genetic differences of Trypanosoma in some Chinese freshwater fishes: difficulties of species identification

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	Morphological and genetic differences of Trypanosoma in some Chinese freshwater fishes: difficulties of species identification
	Gu, Zemao ; Wang, Jianguo ; Li, Ming ; Zhang, Jinyong ; Ke, Xiaoli ; Gong, Xiaoning
刊名	PARASITOLOGY RESEARCH
	2007-08-01
卷号	101 期号:3 页码:723-730
关键词	GADUS-MORHUA L. OKAVANGO DELTA REDESCRIPTION PISCICOLIDAE PHYLOGENY SEQUENCES BOTSWANA ATLANTIC BLOOD COD
ISSN号	0932-0113
通讯作者	Wang, JG, Chinese Acad Sci, Inst Hydrobiol, Res Ctr Fisheries Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Blood smears and purified trypanosome from freshwater fishes yellow catfish (Pseudobagras fulvidraco) and common carp (Cyprinus carpio) captured from Niushan Lake, Hubei Province were examined to determine whether all of their trypanosomes were Trypanosoma pseudobagri, a species of supposed host specificity and widespread existence across China. Trypanosomes occurred in 16/16 blood smears, and morphometric character analysis of trypanosomes from these smears showed that there were three morphospecies, Trypanosoma sp Carpio, T. sp Pseudobagri, and T. sp. 18S rDNA sequences of trypanosomes from 16 samples revealed three genetic groups among these fish trypanosomes. Group 1 was from C. carpio containing T. sp Carpio; groups 2 and 3 were from P. fulvidraco containing T. sp Pseudobagri and T. sp, respectively. The high similarity of morphometric characters and 18S rDNA sequences showed that T. sp Carpio and T. siniperca probably were the same species. T. sp Pseudobagri was the first occurrence in China. Sequence comparison showed that T. sp Pseudobagri sequence was most similar to that of clone Marv, whereas T. sp sequence differ from those of T. sp Carpio and T. sp Pseudobagri by 5.4 and 5.8%, respectively, and tentatively identified as T. pseudobagri. It was concluded that three species of trypanosomes, at least three genotypes occur in Niushan Lake fishes, and P. fulvidraco in this region appear to contain both types, although the identification of T. pseudobagri remains a problem.
英文摘要	Blood smears and purified trypanosome from freshwater fishes yellow catfish (Pseudobagras fulvidraco) and common carp (Cyprinus carpio) captured from Niushan Lake, Hubei Province were examined to determine whether all of their trypanosomes were Trypanosoma pseudobagri, a species of supposed host specificity and widespread existence across China. Trypanosomes occurred in 16/16 blood smears, and morphometric character analysis of trypanosomes from these smears showed that there were three morphospecies, Trypanosoma sp Carpio, T. sp Pseudobagri, and T. sp. 18S rDNA sequences of trypanosomes from 16 samples revealed three genetic groups among these fish trypanosomes. Group 1 was from C. carpio containing T. sp Carpio; groups 2 and 3 were from P. fulvidraco containing T. sp Pseudobagri and T. sp, respectively. The high similarity of morphometric characters and 18S rDNA sequences showed that T. sp Carpio and T. siniperca probably were the same species. T. sp Pseudobagri was the first occurrence in China. Sequence comparison showed that T. sp Pseudobagri sequence was most similar to that of clone Marv, whereas T. sp sequence differ from those of T. sp Carpio and T. sp Pseudobagri by 5.4 and 5.8%, respectively, and tentatively identified as T. pseudobagri. It was concluded that three species of trypanosomes, at least three genotypes occur in Niushan Lake fishes, and P. fulvidraco in this region appear to contain both types, although the identification of T. pseudobagri remains a problem.
学科主题	Parasitology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Parasitology
研究领域[WOS]	Parasitology
关键词[WOS]	GADUS-MORHUA L. ; OKAVANGO DELTA ; REDESCRIPTION ; PISCICOLIDAE ; PHYLOGENY ; SEQUENCES ; BOTSWANA ; ATLANTIC ; BLOOD ; COD
收录类别	SCI
语种	英语
WOS记录号	WOS:000248190500032
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8512]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Res Ctr Fisheries Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Wuhan 430072, Peoples R China 3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Lab Hlth Aquaculture, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Gu, Zemao,Wang, Jianguo,Li, Ming,et al. Morphological and genetic differences of Trypanosoma in some Chinese freshwater fishes: difficulties of species identification[J]. PARASITOLOGY RESEARCH,2007,101(3):723-730.
APA	Gu, Zemao,Wang, Jianguo,Li, Ming,Zhang, Jinyong,Ke, Xiaoli,&Gong, Xiaoning.(2007).Morphological and genetic differences of Trypanosoma in some Chinese freshwater fishes: difficulties of species identification.PARASITOLOGY RESEARCH,101(3),723-730.
MLA	Gu, Zemao,et al."Morphological and genetic differences of Trypanosoma in some Chinese freshwater fishes: difficulties of species identification".PARASITOLOGY RESEARCH 101.3(2007):723-730.