Improvement and extension of the application scope for matrix-assisted laser desorption/ionization mass spectrometric analysis-oriented N-alkylpyridinium isotope quaternization

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	Improvement and extension of the application scope for matrix-assisted laser desorption/ionization mass spectrometric analysis-oriented N-alkylpyridinium isotope quaternization
	Wang H(汪航) ; Wang HY(王昊阳) ; Zhang L(张立) ; Zhang J(张菁) ; Leng JP(冷嘉鹏) ; Cai TT(蔡婷婷) ; Guo YL(郭寅龙)
刊名	Anal. Chim. Acta
	2011
卷号	707 期号:1 页码:100-106
ISSN号	0003-2670
其他题名	N-烷基吡啶同位素季铵化反应结合基质辅助激光解析傅里叶变换质谱分析应用的改进和扩展
通讯作者	郭寅龙
英文摘要	Based on our previous report on N-alkylpyridinium isotope quaternization (NAPIQ) for the analysis of cholesterol and fatty alcohols, we have further developed the NAPIQ method for wider applications in screening various small compounds. The reaction scope and improvement were investigated via the screening of compounds with different reaction sites. The experimental results showed that the NAPIQ strategy was suitable not only for steroids with alcoholic or alpha,beta-unsaturated ketone moieties, but could also be applied to label carbohydrates by changing the reaction solvent. The byproducts from target compounds with multiple groups could be reduced by improving the reaction conditions. The derivatization method significantly improved the detection sensitivity for these compounds in matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometric (MALDI-FTMS) analysis. The detection sensitivity and selectivity of the NAPIQ method were higher than conventionally used Girard reagents. Finally, endogenous steroids in urine samples were screened using the NAPIQ method. NAPIQ was proven to be an efficient alternative method for analyzing steroids and carbohydrates, without the need for isotopically coded internal standards. (C) 2011 Elsevier B.V. All rights reserved.
学科主题	分析化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.aca.2011.09.019
语种	英语
WOS记录号	WOS:000297141800012
公开日期	2013-02-25
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/20060]
专题	上海有机化学研究所_分析化学研究室
推荐引用方式 GB/T 7714	Wang H,Wang HY,Zhang L,et al. Improvement and extension of the application scope for matrix-assisted laser desorption/ionization mass spectrometric analysis-oriented N-alkylpyridinium isotope quaternization[J]. Anal. Chim. Acta,2011,707(1):100-106.
APA	汪航.,王昊阳.,张立.,张菁.,冷嘉鹏.,...&郭寅龙.(2011).Improvement and extension of the application scope for matrix-assisted laser desorption/ionization mass spectrometric analysis-oriented N-alkylpyridinium isotope quaternization.Anal. Chim. Acta,707(1),100-106.
MLA	汪航,et al."Improvement and extension of the application scope for matrix-assisted laser desorption/ionization mass spectrometric analysis-oriented N-alkylpyridinium isotope quaternization".Anal. Chim. Acta 707.1(2011):100-106.