Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC
	Fang, Huaifang ; Yang, Fangxing ; Sun, Junling ; Zeng, Zhaorui ; Xu, Ying
刊名	ELECTROPHORESIS
	2007-10-01
卷号	28 期号:20 页码:3697-3704
关键词	MEKC neutral analyte pressure-assisted field-amplified sample injection
ISSN号	0173-0835
通讯作者	Zeng, ZR, Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
中文摘要	This paper describes a novel method that applies pressure-assisted field-amplified sample injection with reverse migrating micelles (PA-FASI-RMM) for the online concentration of neutral analytes in MEKC with a low-pH BGE. After injection of a plug of water into the separation capillary, negative voltage and positive pressure were simultaneously applied to initialize PA-FASI-RMM injection. The hydrodynamic flow generated by the positive pressure compensated the reverse EOF in the water plug and allowed the water plug to remain in the capillary during FASI with reverse migrating micelles (FASI-RMM) to obtain a much longer injection time than usual, which improved stacking efficiency greatly. Equations describing this injection mode were introduced and were supported by experimental results. For a 450-s online PA-FASI-RMM injection, three orders of magnitude sample enhancement in terms of peak area could be observed for the steroids and an achievement of detection limits was between 1 and 10 ng/mL.
英文摘要	This paper describes a novel method that applies pressure-assisted field-amplified sample injection with reverse migrating micelles (PA-FASI-RMM) for the online concentration of neutral analytes in MEKC with a low-pH BGE. After injection of a plug of water into the separation capillary, negative voltage and positive pressure were simultaneously applied to initialize PA-FASI-RMM injection. The hydrodynamic flow generated by the positive pressure compensated the reverse EOF in the water plug and allowed the water plug to remain in the capillary during FASI with reverse migrating micelles (FASI-RMM) to obtain a much longer injection time than usual, which improved stacking efficiency greatly. Equations describing this injection mode were introduced and were supported by experimental results. For a 450-s online PA-FASI-RMM injection, three orders of magnitude sample enhancement in terms of peak area could be observed for the steroids and an achievement of detection limits was between 1 and 10 ng/mL.
学科主题	Biochemical Research Methods; Chemistry ; Analytical
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	CAPILLARY-ZONE-ELECTROPHORESIS ; SELECTIVE EXHAUSTIVE INJECTION ; STACKING NEUTRAL ANALYTES ; ELECTROKINETIC CHROMATOGRAPHY ; ONLINE CONCENTRATION ; HIGH-SALT ; OPTIMIZATION ; SPECTROMETRY ; ENHANCEMENT ; SEPARATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000250657100014
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8386]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol, Wuhan, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Fang, Huaifang,Yang, Fangxing,Sun, Junling,et al. Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC[J]. ELECTROPHORESIS,2007,28(20):3697-3704.
APA	Fang, Huaifang,Yang, Fangxing,Sun, Junling,Zeng, Zhaorui,&Xu, Ying.(2007).Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC.ELECTROPHORESIS,28(20),3697-3704.
MLA	Fang, Huaifang,et al."Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC".ELECTROPHORESIS 28.20(2007):3697-3704.