Clonal diversity and genealogical relationships of gibel carp in four hatcheries

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	Clonal diversity and genealogical relationships of gibel carp in four hatcheries
	Li, F. -B.; Gui, J. -F.
刊名	ANIMAL GENETICS
	2008-02-01
卷号	39 期号:1 页码:28-33
关键词	clones control region genetic diversity gibel carp mitochondrial DNA transferrin
ISSN号	0268-9146
通讯作者	Gui, JF, Chinese Acad Sci, Inst Hydrobiol, Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China
中文摘要	To conserve and utilize the genetic pool of gynogenetic gibel carp (Carassius auratus gibelio), the Fangzheng and Qihe stock hatcheries have been established in China. However, little information is available on the amount of genetic variation within and between these populations. In this study, clonal diversity in 101 fish from these two stock hatcheries and 35 fish from two other hatcheries in Wuhan and Pengze respectively was analysed for variation in serum transferrin. Thirteen clones were found in Fangzheng and Qihe, of which 12 were novel. Six clones were specific to Fangzheng and three specific to Qihe, whereas four were shared among the Fangzheng and Qihe fish. To obtain more knowledge on genetic diversity and genealogical relationships within gibel carp, the complete mitochondrial DNA (mtDNA) control region (similar to 920 bp) was sequenced in 64 individuals representing all 14 clones identified in the four hatcheries. Differences in the mtDNA sequences varied remarkably among hatcheries, with the Fangzheng and Qihe lines demonstrating high diversity and Wuhan and Pengze showing no variation. The Fangzheng and Qihe lines might represent two distinct matrilineal sources. One of the Qihe samples carried the haplotype shared by a most widely cultivated Fangzheng clone, indicating that a Fangzheng clone escaped from cultivated ponds and moved into the Qihe hatchery. Four Fangzheng samples clustered within the lineage formed mainly by Qihe samples, most likely reflecting historical gene flow from Qihe to Fangzheng. It is suggested that clones in Wuhan originated from Fangzheng, consistent with their introduction history, supporting the hypothesis that gibel carp in Pengze were domesticated from individuals in the Fangzheng hatchery.
英文摘要	To conserve and utilize the genetic pool of gynogenetic gibel carp (Carassius auratus gibelio), the Fangzheng and Qihe stock hatcheries have been established in China. However, little information is available on the amount of genetic variation within and between these populations. In this study, clonal diversity in 101 fish from these two stock hatcheries and 35 fish from two other hatcheries in Wuhan and Pengze respectively was analysed for variation in serum transferrin. Thirteen clones were found in Fangzheng and Qihe, of which 12 were novel. Six clones were specific to Fangzheng and three specific to Qihe, whereas four were shared among the Fangzheng and Qihe fish. To obtain more knowledge on genetic diversity and genealogical relationships within gibel carp, the complete mitochondrial DNA (mtDNA) control region (similar to 920 bp) was sequenced in 64 individuals representing all 14 clones identified in the four hatcheries. Differences in the mtDNA sequences varied remarkably among hatcheries, with the Fangzheng and Qihe lines demonstrating high diversity and Wuhan and Pengze showing no variation. The Fangzheng and Qihe lines might represent two distinct matrilineal sources. One of the Qihe samples carried the haplotype shared by a most widely cultivated Fangzheng clone, indicating that a Fangzheng clone escaped from cultivated ponds and moved into the Qihe hatchery. Four Fangzheng samples clustered within the lineage formed mainly by Qihe samples, most likely reflecting historical gene flow from Qihe to Fangzheng. It is suggested that clones in Wuhan originated from Fangzheng, consistent with their introduction history, supporting the hypothesis that gibel carp in Pengze were domesticated from individuals in the Fangzheng hatchery.
学科主题	Agriculture ; Dairy & Animal Science; Genetics & Heredity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Agriculture, Dairy & Animal Science ; Genetics & Heredity
研究领域[WOS]	Agriculture ; Genetics & Heredity
关键词[WOS]	CARASSIUS-AURATUS-GIBELIO ; SILVER CRUCIAN CARP ; GENETIC-HETEROGENEITY ; GYNOGENETIC CLONES ; SEQUENCE ALIGNMENT ; PLOIDY LEVEL ; BLOCH ; FISH ; DNA ; REPRODUCTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000252804900005
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8296]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Li, F. -B.,Gui, J. -F.. Clonal diversity and genealogical relationships of gibel carp in four hatcheries[J]. ANIMAL GENETICS,2008,39(1):28-33.
APA	Li, F. -B.,&Gui, J. -F..(2008).Clonal diversity and genealogical relationships of gibel carp in four hatcheries.ANIMAL GENETICS,39(1),28-33.
MLA	Li, F. -B.,et al."Clonal diversity and genealogical relationships of gibel carp in four hatcheries".ANIMAL GENETICS 39.1(2008):28-33.