A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples

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	A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples
	Hu, Chenlin 1,2; Gan, Nanqin 1; He, Zhike 3; Song, Lirong 1
刊名	INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
	2008
卷号	88 期号:4 页码:267-277
关键词	microcystins gold nanoparticles chemiluminescence ELISA
ISSN号	0306-7319
通讯作者	Song, LR, CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China
中文摘要	A novel chemiluminescent immunoassay method based on gold nanoparticles was developed for the detection of microcystins (MCs). The immunoassay included three main steps: indirect competitive immunoreaction, oxidative dissolution of gold nanoparticles, and indirect determination for MCs with Au3+-catalysed luminol chemiluminesent system. The method has a wide working range (0.05-10 mu g L-1, r(2) = 0.9914), the limit of detection was determined to be 0.024 mu g L-1, which is much lower than the World Health Organization's proposed guidelines (1 mu g L-1) for drinking-water. The proposed method was applied to MC analysis in natural water and fish tissue samples, and most results in the proposed method were in agreement with the conventional indirect competitive enzyme-linked immunosorbent assay method, which indicated that the new chemiluminescent immunoassay was sensitive, reliable, and suitable for MC analysis in natural water and fish tissue samples.
英文摘要	A novel chemiluminescent immunoassay method based on gold nanoparticles was developed for the detection of microcystins (MCs). The immunoassay included three main steps: indirect competitive immunoreaction, oxidative dissolution of gold nanoparticles, and indirect determination for MCs with Au3+-catalysed luminol chemiluminesent system. The method has a wide working range (0.05-10 mu g L-1, r(2) = 0.9914), the limit of detection was determined to be 0.024 mu g L-1, which is much lower than the World Health Organization's proposed guidelines (1 mu g L-1) for drinking-water. The proposed method was applied to MC analysis in natural water and fish tissue samples, and most results in the proposed method were in agreement with the conventional indirect competitive enzyme-linked immunosorbent assay method, which indicated that the new chemiluminescent immunoassay was sensitive, reliable, and suitable for MC analysis in natural water and fish tissue samples.
学科主题	Chemistry ; Analytical; Environmental Sciences
WOS标题词	Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]	Chemistry, Analytical ; Environmental Sciences
研究领域[WOS]	Chemistry ; Environmental Sciences & Ecology
关键词[WOS]	MASS-SPECTROMETRY ; WATER ; ANTIBODIES ; ALGAE ; ELISA ; QUANTIFICATION ; CYANOBACTERIA ; HEPATOTOXINS ; DEPOSITION ; NODULARIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000253658600004
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8244]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Hu, Chenlin,Gan, Nanqin,He, Zhike,et al. A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples[J]. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY,2008,88(4):267-277.
APA	Hu, Chenlin,Gan, Nanqin,He, Zhike,&Song, Lirong.(2008).A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples.INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY,88(4),267-277.
MLA	Hu, Chenlin,et al."A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples".INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY 88.4(2008):267-277.