Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803

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	Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803
	Yan, Chunlan; Xu, Xudong
刊名	PROGRESS IN NATURAL SCIENCE
	2008-02-10
卷号	18 期号:2 页码:149-153
关键词	FBPase photoautotrophic growth light sensitivity Synechocystis sp.
ISSN号	1002-0071
通讯作者	Xu, XD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	From a random insertion mutant library of Synechocystis sp. PCC 6803, a mutant defective in photoautotrophic growth was obtained. The interrupted gene was identified to be slr2094 (rbpl), which encodes the fructose-1,6-biphosphatase (FBPase)/sedoheptulose-1,7-biphosphatase (SBPase) bifunctional enzyme (F-I). Two other independently constructed slr2094 mutants showed an identical phenotype. The FBPase activity was found to be virtually lacking in an slr2094 mutant, which was sensitive to light under mixotrophic growth conditions. These results indicate that slr2094 is the only active FBPase-encoding gene in this cyanobacterium. Inactivation of photosystem II by interrupting psbB in slr2094 mutant alleviated the sensitiveness to light. This report provides the direct genetic evidence for the essential role of F-I in the photosynthesis of Synechocystis sp. PCC 6803. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
英文摘要	From a random insertion mutant library of Synechocystis sp. PCC 6803, a mutant defective in photoautotrophic growth was obtained. The interrupted gene was identified to be slr2094 (rbpl), which encodes the fructose-1,6-biphosphatase (FBPase)/sedoheptulose-1,7-biphosphatase (SBPase) bifunctional enzyme (F-I). Two other independently constructed slr2094 mutants showed an identical phenotype. The FBPase activity was found to be virtually lacking in an slr2094 mutant, which was sensitive to light under mixotrophic growth conditions. These results indicate that slr2094 is the only active FBPase-encoding gene in this cyanobacterium. Inactivation of photosystem II by interrupting psbB in slr2094 mutant alleviated the sensitiveness to light. This report provides the direct genetic evidence for the essential role of F-I in the photosynthesis of Synechocystis sp. PCC 6803. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
学科主题	Multidisciplinary Sciences
WOS标题词	Science & Technology ; Technology
类目[WOS]	Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]	Materials Science ; Science & Technology - Other Topics
关键词[WOS]	FRUCTOSE 1,6-BISPHOSPHATASE ; SYNECHOCOCCUS-LEOPOLIENSIS ; MOLECULAR CHARACTERIZATION ; HYDROGEN-PEROXIDE ; PHOTOSYSTEM-II ; PCC 6803 ; CHLOROPLAST ; FRUCTOSE-1,6-BISPHOSPHATASE ; PURIFICATION ; PROTEIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000253767200003
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8238]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Yan, Chunlan,Xu, Xudong. Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803[J]. PROGRESS IN NATURAL SCIENCE,2008,18(2):149-153.
APA	Yan, Chunlan,&Xu, Xudong.(2008).Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803.PROGRESS IN NATURAL SCIENCE,18(2),149-153.
MLA	Yan, Chunlan,et al."Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803".PROGRESS IN NATURAL SCIENCE 18.2(2008):149-153.