Avian influenza virus infection induces differential expression of genes in chicken kidney

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Avian influenza virus infection induces differential expression of genes in chicken kidney
	Zhang, Wanpo 1; Li, Huiying 2; Cheng, Guofu 1; Hu, Sishun 1; Li, Zili 1; Bi, Dingren 1
刊名	RESEARCH IN VETERINARY SCIENCE
	2008-06-01
卷号	84 期号:3 页码:374-381
关键词	avian influenza virus (AIV) chicken suppression subtractive hybridization (SSH) gene differential expression
ISSN号	0034-5288
通讯作者	Bi, DG, Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
中文摘要	The pathogenic process of highly pathogenic avian influenza virus (HPAIV) infection is poorly understood. To explore the differential expression of kidney genes as a result of HPAIV infection, two cDNA libraries were constructed from uninfected and infected kidneys by suppression subtractive hybridization (SSH). Fifteen genes including IFN-stimulated genes (ISG12), lymphocyte antigen 6 complex locus E gene (LY6E), matrix Gla protein gene (MGP), lysozyme gene, haemopoiesis related membrane protein I gene, KIAA1259, MGC68696, G6pe-prov protein gene (G6PC), MGC4504, alcohol dehydrogenase gene (ADH), glutathione S-transferase gene (GST), sodium-dependent high-affinity dicarboxylate transporter gene (SDCT), Synaptotagmin XV (SytXV) and two novel genes were found significantly up-regulated or dramatically suppressed. Differential expression of these genes was further identified by Northern blot. Functional analysis indicated that the regulation of their expression might contribute to the pathogenic process of HPAIV infection. In contrast, the increased expression of three IFN-stimulated genes named ISG12, LY6E, and haemopoiesis related membrane protein 1 gene might reflect host defense responses. Further study showed that ISG12 protein failed to directly interact with NS1 protein of HPAIV which expressed simultaneously in the organs where HPAIV replication occurred, by use of BacterioMatch two-hybrid system. Therefore, our findings may provide new insights into understanding the molecular mechanism underlying the pathophysiological process of HPAIV infection in chicken. (c) 2007 Elsevier Ltd. All rights reserved.
英文摘要	The pathogenic process of highly pathogenic avian influenza virus (HPAIV) infection is poorly understood. To explore the differential expression of kidney genes as a result of HPAIV infection, two cDNA libraries were constructed from uninfected and infected kidneys by suppression subtractive hybridization (SSH). Fifteen genes including IFN-stimulated genes (ISG12), lymphocyte antigen 6 complex locus E gene (LY6E), matrix Gla protein gene (MGP), lysozyme gene, haemopoiesis related membrane protein I gene, KIAA1259, MGC68696, G6pe-prov protein gene (G6PC), MGC4504, alcohol dehydrogenase gene (ADH), glutathione S-transferase gene (GST), sodium-dependent high-affinity dicarboxylate transporter gene (SDCT), Synaptotagmin XV (SytXV) and two novel genes were found significantly up-regulated or dramatically suppressed. Differential expression of these genes was further identified by Northern blot. Functional analysis indicated that the regulation of their expression might contribute to the pathogenic process of HPAIV infection. In contrast, the increased expression of three IFN-stimulated genes named ISG12, LY6E, and haemopoiesis related membrane protein 1 gene might reflect host defense responses. Further study showed that ISG12 protein failed to directly interact with NS1 protein of HPAIV which expressed simultaneously in the organs where HPAIV replication occurred, by use of BacterioMatch two-hybrid system. Therefore, our findings may provide new insights into understanding the molecular mechanism underlying the pathophysiological process of HPAIV infection in chicken. (c) 2007 Elsevier Ltd. All rights reserved.
学科主题	Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Veterinary Sciences
研究领域[WOS]	Veterinary Sciences
关键词[WOS]	SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; A VIRUSES ; PROTEIN ; IDENTIFICATION ; H5N1 ; ISG12 ; MICE ; GLUTATHIONE ; PROFILE ; CLONING
收录类别	SCI
语种	英语
WOS记录号	WOS:000255832900010
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8132]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wanpo,Li, Huiying,Cheng, Guofu,et al. Avian influenza virus infection induces differential expression of genes in chicken kidney[J]. RESEARCH IN VETERINARY SCIENCE,2008,84(3):374-381.
APA	Zhang, Wanpo,Li, Huiying,Cheng, Guofu,Hu, Sishun,Li, Zili,&Bi, Dingren.(2008).Avian influenza virus infection induces differential expression of genes in chicken kidney.RESEARCH IN VETERINARY SCIENCE,84(3),374-381.
MLA	Zhang, Wanpo,et al."Avian influenza virus infection induces differential expression of genes in chicken kidney".RESEARCH IN VETERINARY SCIENCE 84.3(2008):374-381.