Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)
Zheng, J. S.1; Liao, X. L.1,2; Tong, J. G.1,2; Du, H. J.1; Milinkovitch, M. C.3; Wang, D.1
刊名CONSERVATION GENETICS
2008
卷号9期号:4页码:1007-1009
关键词Yangtze finless porpoise Neophocoena phocaenoides asiaeorientalis FIASCO microsatellite conservation genetics
ISSN号1566-0621
通讯作者Wang, D, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要The finless porpoise (Neophocaena phocaenoides) is one of the smallest cetacean species widely distributed in the shallow coastal waters of the Indo-Pacific Oceans. The population size of the Yangtze subspecies (N. p. asiaeorientalis) has sharply decreased in the last two decades and access to objective data on its population structure and genetic diversity would be of great assistance for their proper management. Here we report on the isolation of nine polymorphic microsatellite using the "Fast Isolation by AFLP of Sequences Containing repeats" (FIASCO) protocol. Polymorphism was assessed using 30 porpoise individuals randomly sampled in the Yangtze River. The number of alleles per locus varies from 2 to 9, with an average value of 5.56, whereas the ranges of observed and expected heterozygosi ties were 0.300-0.633 (mean 0.496) and 0.473-0.804 (mean 0.659), respectively.
英文摘要The finless porpoise (Neophocaena phocaenoides) is one of the smallest cetacean species widely distributed in the shallow coastal waters of the Indo-Pacific Oceans. The population size of the Yangtze subspecies (N. p. asiaeorientalis) has sharply decreased in the last two decades and access to objective data on its population structure and genetic diversity would be of great assistance for their proper management. Here we report on the isolation of nine polymorphic microsatellite using the "Fast Isolation by AFLP of Sequences Containing repeats" (FIASCO) protocol. Polymorphism was assessed using 30 porpoise individuals randomly sampled in the Yangtze River. The number of alleles per locus varies from 2 to 9, with an average value of 5.56, whereas the ranges of observed and expected heterozygosi ties were 0.300-0.633 (mean 0.496) and 0.473-0.804 (mean 0.659), respectively.
学科主题Biodiversity Conservation; Genetics & Heredity
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biodiversity Conservation ; Genetics & Heredity
研究领域[WOS]Biodiversity & Conservation ; Genetics & Heredity
收录类别SCI
语种英语
WOS记录号WOS:000257420700030
公开日期2010-10-13
内容类型期刊论文
源URL[http://ir.ihb.ac.cn/handle/152342/8078]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Free Univ Brussels, Inst Mol Biol & Med, Lab Evolutionary Genet, B-6041 Gosselies, Belgium
推荐引用方式
GB/T 7714
Zheng, J. S.,Liao, X. L.,Tong, J. G.,et al. Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)[J]. CONSERVATION GENETICS,2008,9(4):1007-1009.
APA Zheng, J. S.,Liao, X. L.,Tong, J. G.,Du, H. J.,Milinkovitch, M. C.,&Wang, D..(2008).Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis).CONSERVATION GENETICS,9(4),1007-1009.
MLA Zheng, J. S.,et al."Development and characterization of polymorphic microsatellite loci in the endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)".CONSERVATION GENETICS 9.4(2008):1007-1009.
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