Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos

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	Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos
	Pei, De-Sheng 1,2; Sun, Yong-Hua 1; Zhu, Zuo-Yan 1
刊名	MOLECULAR BIOLOGY REPORTS
	2009-07-01
卷号	36 期号:6 页码:1375-1380
关键词	Nuclear reprogramming Nuclear transfer Rare minnow (Gobiocypris rarus) Zebrafish (Danio rerio)
ISSN号	0301-4851
通讯作者	Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	A novel gene-K23, differentially expressed in cross-subfamily cloned embryos, was isolated by RACE-PCR technique. It had 2580 base pairs (bp) in length, with a 1,425 bp open reading frame (ORF) encoding a putative protein of 474 amino acids (aa). Bioinformatic analysis indicated that K23 had 22 phosphorylation sites, but it had no signal peptides. Developmental expression analysis in zebrafish showed that K23 transcripts were maternally expressed in ovum and the amount of K23 transcripts increased gradually from zygote to pharyngula period. Subcellular localization analysis revealed that K23 protein was homogeneously distributed both in nuclei and cytoplasm. Taken together, our findings indicate that K23 gene is a novel gene differentially expressed in fish cross-subfamily cloned embryos.
英文摘要	A novel gene-K23, differentially expressed in cross-subfamily cloned embryos, was isolated by RACE-PCR technique. It had 2580 base pairs (bp) in length, with a 1,425 bp open reading frame (ORF) encoding a putative protein of 474 amino acids (aa). Bioinformatic analysis indicated that K23 had 22 phosphorylation sites, but it had no signal peptides. Developmental expression analysis in zebrafish showed that K23 transcripts were maternally expressed in ovum and the amount of K23 transcripts increased gradually from zygote to pharyngula period. Subcellular localization analysis revealed that K23 protein was homogeneously distributed both in nuclei and cytoplasm. Taken together, our findings indicate that K23 gene is a novel gene differentially expressed in fish cross-subfamily cloned embryos.
学科主题	Biochemistry & Molecular Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	PLURIPOTENT STEM-CELLS ; NUCLEAR TRANSFER ; ENUCLEATED EGGS ; IN-VITRO ; ZEBRAFISH ; FIBROBLASTS ; GENERATION ; METHYLATION ; CLONING ; MOUSE
收录类别	SCI
资助信息	National Natural Science Foundation of China [30700607, 30771100]; State Key Fundamental Research of China [2004CB117406]
语种	英语
WOS记录号	WOS:000266454500023
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7712]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Pei, De-Sheng,Sun, Yong-Hua,Zhu, Zuo-Yan. Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos[J]. MOLECULAR BIOLOGY REPORTS,2009,36(6):1375-1380.
APA	Pei, De-Sheng,Sun, Yong-Hua,&Zhu, Zuo-Yan.(2009).Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos.MOLECULAR BIOLOGY REPORTS,36(6),1375-1380.
MLA	Pei, De-Sheng,et al."Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos".MOLECULAR BIOLOGY REPORTS 36.6(2009):1375-1380.