Characterization of the porcine differentially expressed PDK4 gene and association with meat quality

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	Characterization of the porcine differentially expressed PDK4 gene and association with meat quality
	Lan, Jing 1,2; Lei, Ming-Gang 1,2; Zhang, Yi-Bing 3; Wang, Jian-Hua 1,2; Feng, Xiao-Ting 1,2; Xu, De-Quan 1,2; Gui, Jian-Fang 3; Xiong, Yuan-Zhu 1,2
刊名	MOLECULAR BIOLOGY REPORTS
	2009-09-01
卷号	36 期号:7 页码:2003-2010
关键词	Porcine PDK4 Differential gene expression Suppression subtractive hybridization Meat quality
ISSN号	0301-4851
通讯作者	Lei, MG, Huazhong Agr Univ, Minist Agr, Key Lab Swine Genet & Breeding, Wuhan 430070, Peoples R China
中文摘要	To investigate the differential expression of genes in the skeletal muscle between Yorkshire and Chinese indigenous breed Meishan pigs, suppression subtractive hybridization was carried out and many genes were proved to be expressed significantly different in the two breeds. One gene highly expressed in Meishan but lowly expressed in Yorkshire specific library, shared strong homology with human pyruvate dehydrogenase kinase 4 (PDK4). Using semi-quantity and quantity PCR, We confirmed its differential expression between the two breeds. Temporal and spatial expression analysis indicated that porcine PDK4 gene is highly expressed in skeletal muscle and the highest in neonatal pigs. Complete cDNA cloning and sequence analysis revealed that porcine PDK4 gene contains an open reading frame of 1,221 bp. The deduced amino acid sequence showed conservation in evolution. A G/A mutation in intron 9 was identified and association analysis showed that it was significantly associated with intramuscular fat, muscle water content.
英文摘要	To investigate the differential expression of genes in the skeletal muscle between Yorkshire and Chinese indigenous breed Meishan pigs, suppression subtractive hybridization was carried out and many genes were proved to be expressed significantly different in the two breeds. One gene highly expressed in Meishan but lowly expressed in Yorkshire specific library, shared strong homology with human pyruvate dehydrogenase kinase 4 (PDK4). Using semi-quantity and quantity PCR, We confirmed its differential expression between the two breeds. Temporal and spatial expression analysis indicated that porcine PDK4 gene is highly expressed in skeletal muscle and the highest in neonatal pigs. Complete cDNA cloning and sequence analysis revealed that porcine PDK4 gene contains an open reading frame of 1,221 bp. The deduced amino acid sequence showed conservation in evolution. A G/A mutation in intron 9 was identified and association analysis showed that it was significantly associated with intramuscular fat, muscle water content.
学科主题	Biochemistry & Molecular Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	SKELETAL-MUSCLE ; SEQUENCE TAGS ; ERR-ALPHA ; DEHYDROGENASE ; PGC-1-ALPHA ; IDENTIFICATION ; PARENTS ; MEISHAN ; COMPLEX ; FATNESS
收录类别	SCI
资助信息	National Natural Science Foundation of China [30400313]; National Key Foundation Research and Development Program of China [2006CB102102]; National High Technology Research and Development Program of China [2006AA10Z1D6]
语种	英语
WOS记录号	WOS:000268496800047
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7630]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Huazhong Agr Univ, Minist Agr, Key Lab Swine Genet & Breeding, Wuhan 430070, Peoples R China 2.Huazhong Agr Univ, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan 430070, Peoples R China 3.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Lan, Jing,Lei, Ming-Gang,Zhang, Yi-Bing,et al. Characterization of the porcine differentially expressed PDK4 gene and association with meat quality[J]. MOLECULAR BIOLOGY REPORTS,2009,36(7):2003-2010.
APA	Lan, Jing.,Lei, Ming-Gang.,Zhang, Yi-Bing.,Wang, Jian-Hua.,Feng, Xiao-Ting.,...&Xiong, Yuan-Zhu.(2009).Characterization of the porcine differentially expressed PDK4 gene and association with meat quality.MOLECULAR BIOLOGY REPORTS,36(7),2003-2010.
MLA	Lan, Jing,et al."Characterization of the porcine differentially expressed PDK4 gene and association with meat quality".MOLECULAR BIOLOGY REPORTS 36.7(2009):2003-2010.