The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b(5) and mutant V45H

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	The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b(5) and mutant V45H
	Zhang Q(张琪) ; Cao CY(曹春阳) ; Wang ZQ(王子强) ; Wang YH(王韵华) ; Wu HM(吴厚铭) ; Huang ZX(黄仲贤)
刊名	Protein Sci.
	2004
卷号	13 期号:8 页码:2161-2169
ISSN号	0961-8368
其他题名	细胞色素B5及其突变子V45H溶液结构的比较研究
通讯作者	吴厚铭 ; 黄仲贤
英文摘要	A comparative study on the solution structures of bovine microsomal cytochrome b(5) (Tb-5) and the mutant V45H has been achieved by 1D and 2D H-1-NMR spectroscopy to clarify the differences in the solution conformations between these two proteins. The results reveal that the global folding of the V45H mutant in solution is unchanged, but the subtle changes exist in the orientation of the axial ligand His39, and heme vinyl groups. The side chain of His45 in V45H mutant extends to the outer edge of the heme pocket leaving a cavity at the site originally occupied by the inner methyl group of Val45 residue. In addition, the imidazole ring of axial ligand His39 rotates counterclockwise by similar to3degrees around the His-Fe-His axis, and the 4-heme vinyl group turns to the space vacated by the removed side chain due to the mutation. Furthermore, the helix III of the heme pocket undergoes outward displacement, while the linkage between helix II and III is shifted leftward. These observations are not only consistent with the pattern of the pseudocontact shifts of the heme protons, but also well account for the lower stability of V45H mutant against heat and urea.
学科主题	天然产物有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1110/ps.04721104
语种	英语
WOS记录号	WOS:000222928800018
公开日期	2013-02-21
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/17339]
专题	上海有机化学研究所_上海有机化学研究所
推荐引用方式 GB/T 7714	Zhang Q,Cao CY,Wang ZQ,et al. The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b(5) and mutant V45H[J]. Protein Sci.,2004,13(8):2161-2169.
APA	张琪,曹春阳,王子强,王韵华,吴厚铭,&黄仲贤.(2004).The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b(5) and mutant V45H.Protein Sci.,13(8),2161-2169.
MLA	张琪,et al."The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b(5) and mutant V45H".Protein Sci. 13.8(2004):2161-2169.