Studies on the effect of column angle in centrifugal helix counter-current chromatography

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	Studies on the effect of column angle in centrifugal helix counter-current chromatography
	Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
刊名	Journal of Chromatography A
	2010
卷号	1217 期号:14 页码:2117-2122
关键词	Centrifugal counter-current chromatography Angle between column axis and centrifugal force Retention of the stationary phase Resolution Dipeptide DNP-amino acid
ISSN号	219673
英文摘要	The performance of the coiled column of centrifugal counter-current chromatography was investigated by changing the angle between column axis and centrifugal force in the separation of dipeptides or DNP-amino acids each with suitable two-phase solvent systems. In general, retention of the stationary phase (Sf) decreased, and peak resolution (Rs) increased as the column angle was increased. The first series of experiments was performed using a polar two-phase solvent system composed of 1-butanol-acetic acid-water (4:1:5, v/v/v) to separate two dipeptide samples, Trp-Tyr and Val-Tyr, at a flow rate of 1 ml/min at 1000 rpm. When the column angle was changed from 0° to 90°, Rs increased from 1.05 (Sf = 60.1%) to 1.17 (Sf = 38.7%) with the lower phase mobile and from 1.02 (Sf = 67.8%) to 1.14 (Sf = 47.4%) with the upper phase mobile, respectively. The second series of experiments was similarly performed with a more hydrophobic two-phase solvent system composed of hexane-ethyl acetate-methanol-0.1 M hydrochloric acid (1:1:1:1, v/v/v/v) to separate three DNP-amino acids, DNP-glu, DNP-β-ala and DNP-ala, at a flow rate of 1 ml/min at 1000 rpm. When the column angle was changed from 0° to 90°, Rs increased from 1.38 (1st peak/2nd peak) and 1.20 (2nd peak/3rd peak) (Sf = 61.1%) to 1.66 and 1.45 (Sf = 34.4%) with the lower phase mobile and from 1.14 and 0.63 (Sf = 72.2%) to 1.53 and 0.87 (Sf = 51.1%) with the upper phase mobile, respectively. The overall results of our studies indicate that increasing the column angle against the radially acting centrifugal force enhances the mixing of two phases in the column to improve the peak while decreasing the stationary phase retention by interrupting the laminar flow of the mobile phase.
学科主题	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000276285600006
公开日期	2012-11-29
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/1854]
专题	新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室
作者单位	NHLBI, NIH, Bioseparat Technol Lab, Biochem & Biophys Ctr, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China; Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. Studies on the effect of column angle in centrifugal helix counter-current chromatography[J]. Journal of Chromatography A,2010,1217(14):2117-2122.
APA	Yang Yi,Gu Dongyu,Aisa Haji Akber,&Ito Yoichiro.(2010).Studies on the effect of column angle in centrifugal helix counter-current chromatography.Journal of Chromatography A,1217(14),2117-2122.
MLA	Yang Yi,et al."Studies on the effect of column angle in centrifugal helix counter-current chromatography".Journal of Chromatography A 1217.14(2010):2117-2122.