NOVEL DESIGN FOR CENTRIFUGAL COUNTER-CURRENT CHROMATOGRAPHY: III. SAW TOOTH COLUMN | |
Yang Yi; Aisa Haji Akber; Ito Yoichiro | |
刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
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2010 | |
卷号 | 33期号:6页码:846-858 |
关键词 | counter-current chromatography dipeptide DNP-amino acid resolution retention of the stationary phase saw tooth column |
ISSN号 | 10826076 |
英文摘要 | The toroidal coil using an equilateral triangular core and zigzag pattern column have improved both retention of the stationary phase and peak resolution of the conventional toroidal coil in centrifugal counter-current chromatography. To further improve the retention of stationary phase and peak resolution, a novel saw tooth column was designed and the performance of the system was evaluated at various flow rates. The results indicated that both retention of the stationary phase and peak resolution were improved as the flow rate was decreased and at a flow rate of 0.005mL/min the resolution is remarkably increased. Modification of the tubing called flat-twisted tubing further improved the peak resolution without increasing the column pressure. With decreased column length at a capacity of about 0.2mL, resolution of the saw tooth column was 1.02. |
学科主题 | Biochemistry & Molecular Biology ; Chemistry |
收录类别 | SCI |
WOS记录号 | WOS:000277574500012 |
公开日期 | 2012-11-29 |
内容类型 | 期刊论文 |
源URL | [http://ir.xjipc.cas.cn/handle/365002/1775] ![]() |
专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 |
作者单位 | NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Yang Yi,Aisa Haji Akber,Ito Yoichiro. NOVEL DESIGN FOR CENTRIFUGAL COUNTER-CURRENT CHROMATOGRAPHY: III. SAW TOOTH COLUMN[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(6):846-858. |
APA | Yang Yi,Aisa Haji Akber,&Ito Yoichiro.(2010).NOVEL DESIGN FOR CENTRIFUGAL COUNTER-CURRENT CHROMATOGRAPHY: III. SAW TOOTH COLUMN.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,33(6),846-858. |
MLA | Yang Yi,et al."NOVEL DESIGN FOR CENTRIFUGAL COUNTER-CURRENT CHROMATOGRAPHY: III. SAW TOOTH COLUMN".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 33.6(2010):846-858. |
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