A selective and sensitive D-xylose electrochemical biosensor based on xylose dehydrogenase displayed on the surface of bacteria and multi-walled carbon nanotubes modified electrode

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 生物传感技术团队

	A selective and sensitive D-xylose electrochemical biosensor based on xylose dehydrogenase displayed on the surface of bacteria and multi-walled carbon nanotubes modified electrode
	Li, Liang 2,3,4; Liang, Bo 2,3; Shi, Jianguo 5; Li, Feng 4; Mascini, Marco 1; Liu, Aihua 2,3
刊名	BIOSENSORS & BIOELECTRONICS
	2012-03-15
卷号	33 期号:1 页码:100-105
关键词	D-Xylose Electrochemical biosensor Xylose dehydrogenase displayed bacteria Multi-walled carbon nanotubes
中文摘要	A novel Nafion/bacteria-displaying xylose dehydrogenase (XDH)/multi-walled carbon nanotubes (MWNTs) composite film-modified electrode was fabricated and applied for the sensitive and selective determination ofd-xylose (INS 967), where the XDH-displayed bacteria (XDH-bacteria) was prepared using a newly identified ice nucleation protein from Pseudomonas borealis DL7 as an anchoring motif. The XDH-displayed bacteria can be used directly, eliminating further enzyme-extraction and purification, thus greatly improved the stability of the enzyme. The optimal conditions for the construction of biosensor were established: homogeneous Nafion–MWNTs composite dispersion (10 μL) was cast onto the inverted glassy carbon electrode, followed by casting 10-μL of XDH-bacteria aqueous solution to stand overnight to dry, then a 5-μL of Nafion solution (0.05 wt%) is syringed to the electrode surface. The bacteria-displaying XDH could catalyze the oxidization of xylose to xylonolactone with coenzyme NAD⁺ in 0.1 M PBS buffer (pH7.4), where NAD⁺ (nicotinamide adenine dinucleotide) is reduced to NADH (the reduced form of nicotinamide adenine dinucleotide). The resultant NADH is further electrocatalytically oxidized by MWNTs on the electrode, resulting in an obvious oxidation peak around 0.50 V (vs. Ag/AgCl). In contrast, the bacteria-XDH-only modified electrode showed oxidation peak at higher potential of 0.7 V and less sensitivity. Therefore, the electrode/MWNTs/bacteria-XDH/Nafion exhibited good analytical performance such as long-term stability, a wide dynamic range of 0.6–100 μM and a low detection limit of 0.5 μM d-xylose (S/N = 3). No interference was observed in the presence of 300-fold excess of other saccharides including d-glucose, d-fructose, d-maltose, d-galactose, d-mannose, d-sucrose, and d-cellbiose as well as 60-fold excess of l-arabinose. The proposed microbial biosensor is stable, specific, sensitive, reproducible, simple, rapid and cost-effective, which holds great potential in real applications.
英文摘要	A novel Nafion/bacteria-displaying xylose dehydrogenase (XDH)/multi-walled carbon nanotubes (MwNTs) composite film-modified electrode was fabricated and applied for the sensitive and selective determination of D-xylose (INS 967), where the XDH-displayed bacteria (XDH-bacteria) was prepared using a newly identified ice nucleation protein from Pseudomonas borealis DL7 as an anchoring motif. The XDH-displayed bacteria can be used directly, eliminating further enzyme-extraction and purification, thus greatly improved the stability of the enzyme. The optimal conditions for the construction of biosensor were established: homogeneous Nafion-MWNTs composite dispersion (10 mu L) was cast onto the inverted glassy carbon electrode, followed by casting 10-mu L of XDH-bacteria aqueous solution to stand overnight to dry, then a 5-mu L of Nafion solution (0.05 wt%) is syringed to the electrode surface. The bacteria-displaying XDH could catalyze the oxidization of xylose to xylonolactone with coenzyme NAD(+) in 0.1 M PBS buffer (pH7.4), where NAD(+) (nicotinamide adenine dinucleotide) is reduced to NADH (the reduced form of nicotinamide adenine dinucleotide). The resultant NADH is further electrocatalytically oxidized by MWNTs on the electrode, resulting in an obvious oxidation peak around 0.50 V (vs. Ag/AgCl). In contrast, the bacteria-XDH-only modified electrode showed oxidation peak at higher potential of 0.7 V and less sensitivity. Therefore, the electrode/MWNTs/bacteria-XDH/Nafion exhibited good analytical performance such as long-term stability, a wide dynamic range of 0.6-100 mu M and a low detection limit of 0.5 mu M D-xylose (S/N = 3). No interference was observed in the presence of 300-fold excess of other saccharides including D-glucose, D-fructose, D-maltose, D-galactose, D-mannose, is-sucrose, and D-cellbiose as well as 60-fold excess of L-arabinose. The proposed microbial biosensor is stable, specific, sensitive, reproducible, simple, rapid and cost-effective, which holds great potential in real applications. (C) 2012 Elsevier B.V. All rights reserved.
学科主题	生物传感器技术
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
研究领域[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
关键词[WOS]	CAPILLARY-ZONE-ELECTROPHORESIS ; DEPENDENT ALDOSE DEHYDROGENASE ; AMPEROMETRIC ENZYME ELECTRODE ; MOLECULAR-WEIGHT SACCHARIDES ; ICE NUCLEATION PROTEIN ; OLIGOSACCHARIDE DEHYDROGENASE ; GLUCONOBACTER-OXYDANS ; LIQUID-CHROMATOGRAPHY ; ALPHA-AMYLASE ; GLUCOSE
收录类别	SCI
语种	英语
WOS记录号	WOS:000301699800016
公开日期	2012-11-10
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1212]
专题	青岛生物能源与过程研究所_生物传感技术团队
作者单位	1.Univ Florence, Dipartimento Chim, I-50019 Sesto Fiorentino, Italy 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Peoples R China 4.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Peoples R China 5.Shandong Acad Sci, Inst Biol, Key Lab Biosensors Shangdong Prov, Jinan 250014, Peoples R China
推荐引用方式 GB/T 7714	Li, Liang,Liang, Bo,Shi, Jianguo,et al. A selective and sensitive D-xylose electrochemical biosensor based on xylose dehydrogenase displayed on the surface of bacteria and multi-walled carbon nanotubes modified electrode[J]. BIOSENSORS & BIOELECTRONICS,2012,33(1):100-105.
APA	Li, Liang,Liang, Bo,Shi, Jianguo,Li, Feng,Mascini, Marco,&Liu, Aihua.(2012).A selective and sensitive D-xylose electrochemical biosensor based on xylose dehydrogenase displayed on the surface of bacteria and multi-walled carbon nanotubes modified electrode.BIOSENSORS & BIOELECTRONICS,33(1),100-105.
MLA	Li, Liang,et al."A selective and sensitive D-xylose electrochemical biosensor based on xylose dehydrogenase displayed on the surface of bacteria and multi-walled carbon nanotubes modified electrode".BIOSENSORS & BIOELECTRONICS 33.1(2012):100-105.