Butanol production from corncob residue using Clostridium beijerinckii NCIMB 8052 | |
Zhang, W. L.1,2; Liu, Z. Y.1; Liu, Z.1; Li, F. L.1 | |
刊名 | LETTERS IN APPLIED MICROBIOLOGY |
2012-09-01 | |
卷号 | 55期号:3页码:240-246 |
关键词 | Butanol Ca(OH)2 detoxification Clostridium beijerinckii NCIMB 8052 corncob residue |
中文摘要 | Methods and Results: In this study, Ca(OH)2 detoxification technique was used to remove inhibitors of lignocellulose enzymatic hydrolysis. During fermentation of untreated corncob residue hydrolysate (CCRH) by Clostridium beijerinckii NCIMB 8052, cell growth was inhibited and only 3·8 g l−1 acetone, butanol and ethanol (ABE) was produced. After pretreatment with Ca(OH)2, enzymatic hydrolysis of CCR resulted in 49·3 g l−1 total sugars, about twofold of that of untreated one. In the fermentation of the Ca(OH)2-detoxified CCRH, sugar utilization ratio was increased by 27·3%. When using the Ca(OH)2-detoxified CCRH supplemented with 10 g l−1 glucose, 16·0 g l−1 ABE was produced, resulting in an ABE yield of 0·32 and a productivity of 0·33 g l−1 h−1. Conclusion: The results in this study suggest that CCR was a good carbon source for ABE fermentation. Significance and Impact of the Study: It is the first time to use CCR as substrate for butanol production. Ca(OH)2 detoxification pretreatment was proved to be an effective method to improve enzymatic digestibility of lignocellulose. |
英文摘要 | Aims: To determine whether corncob residue (CCR) could be a good substrate for butanol production. Methods and Results: In this study, Ca(OH)2 detoxification technique was used to remove inhibitors of lignocellulose enzymatic hydrolysis. During fermentation of untreated corncob residue hydrolysate (CCRH) by Clostridium beijerinckii NCIMB 8052, cell growth was inhibited and only 3.8 g l-1 acetone, butanol and ethanol (ABE) was produced. After pretreatment with Ca(OH)2, enzymatic hydrolysis of CCR resulted in 49.3 g l-1 total sugars, about twofold of that of untreated one. In the fermentation of the Ca(OH)2-detoxified CCRH, sugar utilization ratio was increased by 27.3%. When using the Ca(OH)2-detoxified CCRH supplemented with 10 g l-1 glucose, 16.0 g l-1 ABE was produced, resulting in an ABE yield of 0.32 and a productivity of 0.33 g l-1 h-1. Conclusion: The results in this study suggest that CCR was a good carbon source for ABE fermentation. Significance and Impact of the Study: It is the first time to use CCR as substrate for butanol production. Ca(OH)2 detoxification pretreatment was proved to be an effective method to improve enzymatic digestibility of lignocellulose. |
学科主题 | 微生物资源 |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
关键词[WOS] | CHEMICAL PRETREATMENT METHODS ; ETHANOL-PRODUCTION ; CELLULASE PRODUCTION ; LIME PRETREATMENT ; FERMENTATION ; DETOXIFICATION ; ACID ; BAGASSE ; BA101 ; LIGNOCELLULOSE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000307719200010 |
公开日期 | 2012-11-11 |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1237] |
专题 | 青岛生物能源与过程研究所_微生物资源团队 |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, W. L.,Liu, Z. Y.,Liu, Z.,et al. Butanol production from corncob residue using Clostridium beijerinckii NCIMB 8052[J]. LETTERS IN APPLIED MICROBIOLOGY,2012,55(3):240-246. |
APA | Zhang, W. L.,Liu, Z. Y.,Liu, Z.,&Li, F. L..(2012).Butanol production from corncob residue using Clostridium beijerinckii NCIMB 8052.LETTERS IN APPLIED MICROBIOLOGY,55(3),240-246. |
MLA | Zhang, W. L.,et al."Butanol production from corncob residue using Clostridium beijerinckii NCIMB 8052".LETTERS IN APPLIED MICROBIOLOGY 55.3(2012):240-246. |
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