An untapped bacterial cellulolytic community enriched from coastal marine sediment under anaerobic and thermophilic conditions | |
Ji, Shiqi1; Wang, Shian1; Tan, Yang1; Chen, Xiaohua1; Schwarz, Wolfgang2; Li, Fuli1 | |
刊名 | FEMS MICROBIOLOGY LETTERS |
2012-10-01 | |
卷号 | 335期号:1页码:39-46 |
关键词 | marine anaerobe thermophilic bacteria cellulose degradation cellulase |
中文摘要 | A bacterial community with strong cellulose [filter paper (FP) and microcrystalline cellulose] degradation ability was isolated from the coastal marine environment. They were isolated under thermophilic (60 °C) and anaerobic cultivation conditions. The library of 16S rRNA gene clones revealed a total of 16 operational taxonomic units after 50 clones were surveyed. Sixty percent of the clones were most related to the type strain of Clostridium thermocellum with 16S rRNA gene identity around 87-89%. All of them showed extremely low sequence similarities and were novel at least in species level. The gene clone libraries of glycosyl hydrolase family 48 showed low gene and amino acid sequence similarities around 70-72%. The results indicated that the cellulose degradation systems in the specific environment have not been well studied. The enrichment could disrupt FP within 3 days in a basal medium. The cellulase activity of the community was comparable to that of C. thermocellum LQR1. The main fermentation products were ethanol, acetic acid and butyric acid. This work identified a novel microbial resource with a potential in lignocellulose conversion and biofuel production. |
英文摘要 | A bacterial community with strong cellulose [filter paper (FP) and microcrystalline cellulose] degradation ability was isolated from the coastal marine environment. They were isolated under thermophilic (60 degrees C) and anaerobic cultivation conditions. The library of 16S rRNA gene clones revealed a total of 16 operational taxonomic units after 50 clones were surveyed. Sixty percent of the clones were most related to the type strain of Clostridium thermocellum with 16S rRNA gene identity around 8789%. All of them showed extremely low sequence similarities and were novel at least in species level. The gene clone libraries of glycosyl hydrolase family 48 showed low gene and amino acid sequence similarities around 7072%. The results indicated that the cellulose degradation systems in the specific environment have not been well studied. The enrichment could disrupt FP within 3days in a basal medium. The cellulase activity of the community was comparable to that of C.thermocellum LQR1. The main fermentation products were ethanol, acetic acid and butyric acid. This work identified a novel microbial resource with a potential in lignocellulose conversion and biofuel production. |
学科主题 | 微生物资源 |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Microbiology |
研究领域[WOS] | Microbiology |
关键词[WOS] | CLOSTRIDIUM-THERMOCELLUM ; CRYSTALLINE CELLULOSE ; SP. NOV. ; SEQUENCE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000308582400006 |
公开日期 | 2012-11-11 |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1236] |
专题 | 青岛生物能源与过程研究所_微生物资源团队 |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China 2.Tech Univ Munich, Lehrstuhl Mikrobiol, Freising Weihenstephan, Germany |
推荐引用方式 GB/T 7714 | Ji, Shiqi,Wang, Shian,Tan, Yang,et al. An untapped bacterial cellulolytic community enriched from coastal marine sediment under anaerobic and thermophilic conditions[J]. FEMS MICROBIOLOGY LETTERS,2012,335(1):39-46. |
APA | Ji, Shiqi,Wang, Shian,Tan, Yang,Chen, Xiaohua,Schwarz, Wolfgang,&Li, Fuli.(2012).An untapped bacterial cellulolytic community enriched from coastal marine sediment under anaerobic and thermophilic conditions.FEMS MICROBIOLOGY LETTERS,335(1),39-46. |
MLA | Ji, Shiqi,et al."An untapped bacterial cellulolytic community enriched from coastal marine sediment under anaerobic and thermophilic conditions".FEMS MICROBIOLOGY LETTERS 335.1(2012):39-46. |
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