Spectroscopic investigations of hematoxylin-Eu(III) complex interacting with Herring-sperm DNA

	Spectroscopic investigations of hematoxylin-Eu(III) complex interacting with Herring-sperm DNA
	Huang, Jianhang ; Wang, Xingming ; Ding, Lisheng
刊名	JOURNAL OF COORDINATION CHEMISTRY
	2011
卷号	64 期号:16 页码:2791-2803
关键词	Spectroscopy Hematoxylin Eu(III) Herring-sperm DNA Interaction
ISSN号	0095-8972
通讯作者	Wang, XM (reprint author), SW Univ Sci & Technol, Sch Mat Sci & Engn, Mianyang 621010, Peoples R China.
产权排序	2
英文摘要	The interaction of HE-Eu(III) complex (HE = hematoxylin) with Herring-sperm DNA (hsDNA) has been studied by absorption spectra, fluorescence, and viscosity measurements in physiological buffer (pH = 7.40). The binding constant of HE-Eu(III) complex to hsDNA was obtained by double reciprocal method at 298 and 310K and the corresponding thermodynamic parameters (Delta(r)Hm(circle minus) = 8.55 x 10(4) J mol(-1), Delta(r)Gm(circle minus) = -3.01 x 10(4) J mol(-1), Delta Sm-r(circle minus) = 387.95 J mol(-1) K-1) were calculated, showing that the interaction between HE-Eu(III) complex and hsDNA was driven mainly by entropy. The value of K indicated that the binding mode of HE-Eu(III) complex with DNA was not classical intercalation. These results were further supported by viscosity method and competitive binding experiment. Scatchard analysis suggests that the interaction mode was a mixed binding, which contains partial intercalation and groove binding.
学科主题	Chemistry
收录类别	SCI
资助信息	National Natural Science Foundation of China[30572254]; Postgraduate Innovation Foundation of Southwest University of Science and Technology
语种	英语
WOS记录号	WOS:000299469900003
公开日期	2012-11-09
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/23577]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Huang, Jianhang,Wang, Xingming,Ding, Lisheng. Spectroscopic investigations of hematoxylin-Eu(III) complex interacting with Herring-sperm DNA[J]. JOURNAL OF COORDINATION CHEMISTRY,2011,64(16):2791-2803.
APA	Huang, Jianhang,Wang, Xingming,&Ding, Lisheng.(2011).Spectroscopic investigations of hematoxylin-Eu(III) complex interacting with Herring-sperm DNA.JOURNAL OF COORDINATION CHEMISTRY,64(16),2791-2803.
MLA	Huang, Jianhang,et al."Spectroscopic investigations of hematoxylin-Eu(III) complex interacting with Herring-sperm DNA".JOURNAL OF COORDINATION CHEMISTRY 64.16(2011):2791-2803.