HPLC Analysis of Flavonoids in Lamiophlomisrotata

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	HPLC Analysis of Flavonoids in Lamiophlomisrotata
	Jiang SX(蒋生祥); Liu X(刘霞); Jiang SX(蒋生祥)
刊名	Acta Chromatographica,
	2008
卷号	20 期号:2 页码:247-257
ISSN号	0231-2522
通讯作者	蒋生祥
中文摘要	A simple and precise high-performance liquid chromatographic (HPLC) method has been developed for analysis of flavonoids in Lamiophlomis rotata. HPLC conditions were optimized. Baseline separation was achieved by use of a binary gradient prepared from 100:0.2 (v/v) water–trifluoroacetic acid (component A), and acetonitrile (component B). The gradient was from 20 to 35% B in 10 min, hold at 35% B for 20 min, then from 35 to 70% B in 10 min. The injection volume was 10 μL, and the detection wavelength was 366 nm. Linearity and precision were investigated. When normal leastsquares linear regression (LSLR) was compared with weighted least-squares linear regression (WLSLR), values obtained by use of the latter were much closer to the actual values, over the whole range of concentration, than those obtained by use of the former. WLSLR was therefore used in this work. This HPLC method was successfully used for analysis of the flavonoids in Lamiophlomis rotata and could be effective for quality control of the plant.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the National Natural Science Foundation of China for financial support, no. 20775084.
语种	英语
公开日期	2012-10-11
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/1386]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Jiang SX(蒋生祥); Jiang SX(蒋生祥)
推荐引用方式 GB/T 7714	Jiang SX,Liu X,Jiang SX. HPLC Analysis of Flavonoids in Lamiophlomisrotata[J]. Acta Chromatographica,,2008,20(2):247-257.
APA	蒋生祥,刘霞,&蒋生祥.(2008).HPLC Analysis of Flavonoids in Lamiophlomisrotata.Acta Chromatographica,,20(2),247-257.
MLA	蒋生祥,et al."HPLC Analysis of Flavonoids in Lamiophlomisrotata".Acta Chromatographica, 20.2(2008):247-257.