Saliva microbiomes distinguish caries-active from healthy human populations | |
Yang, Fang1,2,3; Zeng, Xiaowei4; Ning, Kang4; Liu, Kuan-Liang5; Lo, Chien-Chi5; Wang, Wei4; Chen, Jie4; Wang, Dongmei4; Huang, Ranran4; Chang, Xingzhi4 | |
刊名 | ISME JOURNAL |
2012 | |
卷号 | 6期号:1页码:1-10 |
关键词 | caries metagenomics oral-microbiome Prevotella saliva |
中文摘要 | The etiology of dental caries remains elusive because of our limited understanding of the complex oral microbiomes. The current methodologies have been limited by insufficient depth and breadth of microbial sampling, paucity of data for diseased hosts particularly at the population level, inconsistency of sampled sites and the inability to distinguish the underlying microbial factors. By cross-validating 16S rRNA gene amplicon-based and whole-genome-based deep-sequencing technologies, we report the most in-depth, comprehensive and collaborated view to date of the adult saliva microbiomes in pilot populations of 19 caries-active and 26 healthy human hosts. We found that: first, saliva microbiomes in human population were featured by a vast phylogenetic diversity yet a minimal organismal core; second, caries microbiomes were significantly more variable in community structure whereas the healthy ones were relatively conserved; third, abundance changes of certain taxa such as overabundance of Prevotella Genus distinguished caries microbiota from healthy ones, and furthermore, caries-active and normal individuals carried different arrays of Prevotella species; and finally, no 'caries-specific' operational taxonomic units (OTUs) were detected, yet 147 OTUs were 'caries associated', that is, differentially distributed yet present in both healthy and caries-active populations. These findings underscored the necessity of species- and strain-level resolution for caries prognosis, and were consistent with the ecological hypothesis where the shifts in community structure, instead of the presence or absence of particular groups of microbes, underlie the cariogenesis. |
英文摘要 | The etiology of dental caries remains elusive because of our limited understanding of the complex oral microbiomes. The current methodologies have been limited by insufficient depth and breadth of microbial sampling, paucity of data for diseased hosts particularly at the population level, inconsistency of sampled sites and the inability to distinguish the underlying microbial factors. By cross-validating 16S rRNA gene amplicon-based and whole-genome-based deep-sequencing technologies, we report the most in-depth, comprehensive and collaborated view to date of the adult saliva microbiomes in pilot populations of 19 caries-active and 26 healthy human hosts. We found that: first, saliva microbiomes in human population were featured by a vast phylogenetic diversity yet a minimal organismal core; second, caries microbiomes were significantly more variable in community structure whereas the healthy ones were relatively conserved; third, abundance changes of certain taxa such as overabundance of Prevotella Genus distinguished caries microbiota from healthy ones, and furthermore, caries-active and normal individuals carried different arrays of Prevotella species; and finally, no 'caries-specific' operational taxonomic units (OTUs) were detected, yet 147 OTUs were 'caries associated', that is, differentially distributed yet present in both healthy and caries-active populations. These findings underscored the necessity of species-and strain-level resolution for caries prognosis, and were consistent with the ecological hypothesis where the shifts in community structure, instead of the presence or absence of particular groups of microbes, underlie the cariogenesis. The ISME Journal (2012) 6, 1-10; doi:10.1038/ismej.2011.71; published online 30 June 2011 |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Ecology ; Microbiology |
研究领域[WOS] | Environmental Sciences & Ecology ; Microbiology |
关键词[WOS] | CHILDHOOD CARIES ; ORAL MICROFLORA ; DENTAL-CARIES ; HUMAN-BODY ; COMMUNITY ; DIVERSITY ; CHILDREN ; BACTERIA ; DISEASES ; SYSTEM |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000299052500001 |
公开日期 | 2012-11-06 |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1081] |
专题 | 青岛生物能源与过程研究所_单细胞中心 |
作者单位 | 1.Sun Yat Sen Univ, Dept Operat Dent & Endodont, Guanghua Sch, Guangzhou 510155, Guangdong, Peoples R China 2.Sun Yat Sen Univ, Hosp Stomatol, Guangzhou 510155, Guangdong, Peoples R China 3.Sun Yat Sen Univ, Inst Stomatol Res, Guangzhou 510155, Guangdong, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China 5.Los Alamos Natl Lab, Biosci Div, Los Alamos, NM USA |
推荐引用方式 GB/T 7714 | Yang, Fang,Zeng, Xiaowei,Ning, Kang,et al. Saliva microbiomes distinguish caries-active from healthy human populations[J]. ISME JOURNAL,2012,6(1):1-10. |
APA | Yang, Fang.,Zeng, Xiaowei.,Ning, Kang.,Liu, Kuan-Liang.,Lo, Chien-Chi.,...&JianXu.(2012).Saliva microbiomes distinguish caries-active from healthy human populations.ISME JOURNAL,6(1),1-10. |
MLA | Yang, Fang,et al."Saliva microbiomes distinguish caries-active from healthy human populations".ISME JOURNAL 6.1(2012):1-10. |
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