Activity- and Ca2+-dependent modulation of surface expression of brain-derived neurotrophic factor receptors in hippocampal neurons | |
Du, J ; Feng, LY ; Yang, F ; Lu, B | |
刊名 | JOURNAL OF CELL BIOLOGY |
2000 | |
卷号 | 150期号:6页码:1423-1433 |
关键词 | TrkB receptors tetanic stimulation calcium influx Ca2+/calmodulin-dependent kinase II synaptic transmission NERVE GROWTH-FACTOR LONG-TERM POTENTIATION CORTICAL DENDRITIC GROWTH BDNF KNOCKOUT MICE SYNAPTIC TRANSMISSION VISUAL-CORTEX CREB PHOSPHORYLATION GENE-EXPRESSION RAT HIPPOCAMPUS CA1 REGION |
ISSN号 | 0021-9525 |
通讯作者 | Lu, B (reprint author), NICHHD, Unit Synapse Dev & Plast, Dev Neurobiol Lab, NIH, Bldg 49,Rom 5A38,49 Convent Dr, Bethesda, MD 20892 USA, |
英文摘要 | Brain-derived neurotrophic factor (BDNF) has been shown to regulate neuronal survival and synaptic plasticity in the central nervous system (CNS) in an activity-dependent manner, but the underlying mechanisms remain unclear, Here we report that the number of BDNF receptor TrkB on the surface of hippocampal neurons can be enhanced by high frequency neuronal activity and synaptic transmission, and this effect is mediated by Ca2+ influx. Using membrane protein biotinylation as well as receptor binding assays, we show that field electric stimulation increased the number of TrkB on the surface of cultured hippocampal neurons. Immunofluorescence staining suggests that the electric stimulation facilitated the movement of TrkB from intracellular pool to the cell surface, particularly on neuronal processes. The number of surface TrkB was regulated only by high frequency tetanic stimulation, but not by low frequency stimulation. The activity de pendent modulation appears to require Ca2+ influx, since treatment of the neurons with blockers of voltage-gated Ca-2+ channels or NMDA receptors, or removal of extracellular Ca2+, severely attenuated the effect of electric stimulation. Moreover, inhibition of Ca2+/calmodulin-dependent kinase II (CaMKII) significantly reduced the effectiveness of the tetanic stimulation. These findings may help us to understand the role of neuronal activity in neurotrophin function and the mechanism for receptor tyrosine kinase signaling. |
学科主题 | Cell Biology |
收录类别 | SCI |
语种 | 英语 |
公开日期 | 2012-07-23 |
内容类型 | 期刊论文 |
源URL | [http://ir.sibs.ac.cn/handle/331001/2085] |
专题 | 上海神经科学研究所_神经所(总) |
推荐引用方式 GB/T 7714 | Du, J,Feng, LY,Yang, F,et al. Activity- and Ca2+-dependent modulation of surface expression of brain-derived neurotrophic factor receptors in hippocampal neurons[J]. JOURNAL OF CELL BIOLOGY,2000,150(6):1423-1433. |
APA | Du, J,Feng, LY,Yang, F,&Lu, B.(2000).Activity- and Ca2+-dependent modulation of surface expression of brain-derived neurotrophic factor receptors in hippocampal neurons.JOURNAL OF CELL BIOLOGY,150(6),1423-1433. |
MLA | Du, J,et al."Activity- and Ca2+-dependent modulation of surface expression of brain-derived neurotrophic factor receptors in hippocampal neurons".JOURNAL OF CELL BIOLOGY 150.6(2000):1423-1433. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论